The use of appropriate fluorescent proteins has allowed the use of FRET microscopy for investigation of intermolecular interactions in living cells. This method has the advantage of both being dynamic and of working at the subcellular level, so that the time and place where proteins interact can be visualized. We have used FRET microscopy to analyze the interactions between the T cell antigen receptor and the coreceptors CD4 and CD8. This chapter reviews data on how these coreceptors are recruited to the immunological synapse, and how they interact when the T cell is stimulated by different ligands.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079427 | PMC |
| http://dx.doi.org/10.1007/978-3-540-93864-4_2 | DOI Listing |
Publication Analysis
Top Keywords
intermolecular interactions
8
fret microscopy
8
visualizing intermolecular
4
interactions cells
4
cells appropriate
4
appropriate fluorescent
4
fluorescent proteins
4
proteins allowed
4
allowed fret
4
microscopy investigation
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!