The use of phospholipid vesicles (liposomes) in EKC and CEC, as well as the use of CE for studying liposomes and lipid-analyte aggregates, are briefly reviewed. The works are presented in the order of appearance. The review describes the most common liposome dispersions used for achieving separation of neutral and charged compounds in liposome EKC. The CEC part is divided into two parts, discriminating between liposomes immobilized on capillaries for hindering analytes from interacting with the fused-silica wall and liposomes immobilized on capillaries for achieving chromatographic phases for separation of compounds. Finally, the use of CE for studying liposomes and lipid-analyte aggregates as analytes is discussed in brief.
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http://dx.doi.org/10.1002/elps.200900061 | DOI Listing |
Molecules
June 2022
Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.
It is now more than 25 years since the first report of enantioselective analysis by capillary electrophoresis-mass spectrometry (CE-MS) appeared. This article reviews the power of chiral CE-MS in resolving issues on the use of chiral selector incompatibility with MS and poor detectability encountered for chiral compounds by UV detection. The review begins with the general principles, requirements, and critical aspects of chiral CE-MS instrumentation.
View Article and Find Full Text PDFSe Pu
June 2022
Faculty of Chemistry, Northeast Normal University, Changchun 130024, China.
Most drugs used to treat diseases are chiral compounds. Drug enantiomers possess similar physical and chemical properties but may feature distinct pharmacological activities. Drug enantiomers may also exhibit different or even opposite functionalities for metabolism, in terms of the metabolic rate and toxicity in the body.
View Article and Find Full Text PDFElectrophoresis
January 2022
RAM Software Solutions, Tucson, Arizona, USA.
The transport of components in liquid media under the influence of an applied electric field can be described with the continuity equation. It represents a nonlinear conservation law that is based upon the balance laws of continuous transport processes and can be solved in time and space numerically. This procedure is referred to as dynamic computer simulation.
View Article and Find Full Text PDFSe Pu
September 2020
School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
Chiral analysis has been an important research field in modern separation science because the enantiomers of a racemic compound often show different or even opposite bioactivities. A variety of analytical techniques have been adopted for chiral analysis over the past few decades. In comparison with conventional chromatographic methods (e.
View Article and Find Full Text PDFElectrophoresis
January 2021
Division of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Amsterdam Institute for Molecular and Life Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Enantioseparation of chiral products has become increasingly important in a large diversity of academic and industrial applications. The separation of chiral compounds is inherently challenging and thus requires a suitable analytical technique that can achieve high resolution and sensitivity. In this context, CE has shown remarkable results so far.
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