An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a beta-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (approximately 170 kD) showed the ability to transform ginsenoside Rf into Rh(1).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254368 | PMC |
| http://dx.doi.org/10.3390/molecules14062043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ginsenoside Rh1 regulates the immune microenvironment of hepatocellular carcinoma via the glucocorticoid receptor.
J Integr Med
November 2024
Department of Traditional Chinese Medicine, PLA Navy No. 971 Hospital, Qingdao 266071, Shandong Province, China. Electronic address:
Article Synopsis
- Ginsenoside Rh1 (G-Rh1) has potential therapeutic effects on hepatocellular carcinoma (HCC), although its impact was previously unclear, as it has shown effectiveness against other cancers like breast and colon cancer.
- The study used various methods, including bioinformatics and in vitro & in vivo experiments, to examine G-Rh1's effects on HCC cells and the immune environment of tumors.
- Results indicated that G-Rh1 did not significantly impact HCC cell growth but improved the immune response by enhancing the infiltration of key immune cells and increasing MHC-I expression, suggesting its role in fighting cancer by modifying the tumor microenvironment.
Revealing the Mechanisms of Qilongtian Capsules in the Treatment of Chronic Obstructive Pulmonary Disease Based on Integrated Network Pharmacology, Molecular Docking, and Experiments.
ACS Omega
July 2024
Department of Pharmacy, Kunming Municipal Hospital of Traditional Chinese Medicine, Kunming 650011, China.
The Qilongtian capsule (QLT) is a Chinese patent medicine that has been approved for the treatment of chronic obstructive pulmonary disease (COPD). However, the precise pharmacodynamic material basis and molecular mechanism have not been well illustrated. In this study, we identified the effect of QLT on COPD through a cigarette smoke extract (CSE)/lipopolysaccharide (LPS) induced COPD mice model.
View Article and Find Full Text PDFUnveiling the Potential of Migrasomes: A Machine-Learning-Driven Signature for Diagnosing Acute Myocardial Infarction.
Biomedicines
July 2024
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Background: Recent studies have demonstrated that the migrasome, a newly functional extracellular vesicle, is potentially significant in the occurrence, progression, and diagnosis of cardiovascular diseases. Nonetheless, its diagnostic significance and biological mechanism in acute myocardial infarction (AMI) have yet to be fully explored.
Methods: To remedy this gap, we employed an integrative machine learning (ML) framework composed of 113 ML combinations within five independent AMI cohorts to establish a predictive migrasome-related signature (MS).
Quantification of binding capacity of natural products to target proteins by sensors integrating SERS labeling and photocrosslinked molecular probes.
Anal Chim Acta
August 2024
College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou, 310014, China. Electronic address:
Natural products-based screening of active ingredients and their interactions with target proteins is an important ways to discover new drugs. Assessing the binding capacity of target proteins, particularly when multiple components are involved, presents a significant challenge for sensors. As far as we know, there is currently no sensor that can accomplish high-throughput quantitative analysis of natural product-target protein binding capacity based on Raman spectroscopy.
View Article and Find Full Text PDFComprehensive Investigation of Ginsenosides in the Steamed with Different Processing Conditions Using LC-MS.
Molecules
January 2024
Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
(PQ) has been widely used in traditional Chinese medicine and functional food. Ginsenosides are the important functional components of PQ. The ginsenosides' diversity is deeply affected by the processing conditions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!