AI Article Synopsis
- The study compares the Sperm Chromatin Dispersion (SCD) test and the TUNEL assay for assessing DNA fragmentation in human sperm using bright-field microscopy.
- Both tests were applied to semen samples from 60 infertile men and 30 fertile sperm donors, revealing similar amounts of DNA fragmentation.
- The SCD test demonstrated higher sensitivity in detecting sperm DNA damage compared to the TUNEL assay, as some sperm showed damage with SCD but not with TUNEL.
Article Abstract
Objective: To compare sperm chromatin dispersion (SCD) test with terminal deoxynucleotidyle transferase-mediated terminal uridine nick-end labeling (TUNEL) assay in assessing DNA fragmentation in human sperm through bright-field microscopy.
Design: Prospectively designed, side-by-side comparative study.
Setting: Medical genetics laboratory in a provincial research institution.
Patient(s): Sixty male patients presented for infertility evaluation and 30 fertile, volunteer sperm donors.
Intervention(s): Semen analysis, SCD test, and TUNEL assay on the same semen sample and on the same spermatozoa.
Main Outcome Measure(s): Sperm DNA fragmentation, determined by SCD test score or TUNEL assay score.
Result(s): Sperm chromatin dispersion test and TUNEL assay identified similar proportions of sperm cells with DNA fragmentation in the same semen samples. When the SCD test and TUNEL assay were performed simultaneously on the same spermatozoa, TUNEL-negative sperm showed a large halo, whereas TUNEL-positive sperm showed no halo after Diff-Quik staining. However, in some sperm cells, DNA damage was detected by the SCD test but not by the TUNEL assay.
Conclusion(s): Sperm chromatin dispersion test and TUNEL assay are both effective in detecting sperm DNA damage. Using bright-field microscopy, the SCD test appears to be more sensitive than the TUNEL assay.
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| http://dx.doi.org/10.1016/j.fertnstert.2009.04.034 | DOI Listing |
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