Diatoms significantly differ from other groups of heterokont algae in the diversity of chloroplast forms, their number, and location in a cell and in the structure of pyrenoids. The information on the fine structure of chloroplast is recognized to be important for taxonomic and phylogenetic studies of diatoms. Six species of diatoms belonging to different classes have been examined using transmission electron microscopy. New data on the structure of chloroplast have been obtained and characteristics of pyrenoid ultrastructure of diatoms belonging to different phylogenetic clades have been specified. The results enlarge the data obtained earlier and specify a set of features of chloroplast ultrastructure for different phylogenetic clades of diatoms.
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Sci Rep
December 2024
Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, 30 Bolshoy Boulevard, bld. 1, Moscow, 121205, Russia.
Photonic-based methods are crucial in biology and medicine due to their non-invasive nature, allowing remote measurements without affecting biological specimens. The study of diatoms using advanced photonic methods remains a relatively underexplored area, presenting significant opportunities for pioneering discoveries. This research provides a comprehensive analysis of marine diatoms, specifically Nitzschia sp.
View Article and Find Full Text PDFMicrosc Res Tech
December 2024
School of Life Sciences, Xiamen University, Xiamen, China.
Diatoms (Bacillariophyceae) are diverse and abundant microalgae, and many of them are found in marine intertidal environments. Here, we present a new marine epizoic diatom isolated from Euspira gilva Philippi in intertidal area of Beihai city, Guangxi Zhuang Autonomous Region, named Ceratanaulus alaris L.J.
View Article and Find Full Text PDFMolecular data is provided firstly for the newly erected genus Qinia, and the phylogenetic position of the genus Qinia within the Cymbellales is discussed. Despite the presence of apical pore fields bisected by the distal raphe fissure being a very distinctive feature for Qinia, molecular analysis demonstrates this character as homoplasious, having evolved independently in Qinia and Cymbella. Two new species, Qinia hubeii sp.
View Article and Find Full Text PDFJ Phycol
December 2024
Meise Botanic Garden, Research Department, Meise, Belgium.
Scanning electron microscopy has revealed variation in the ultrastructure of distinctive isolated pores in or near the central area of raphid diatoms, with different types of pores being restricted to phylogenetic groups. Thus, the widespread use of the term stigma for all such pores not only hides the structural diversity but also obscures the phylogenetic distribution of the different types. This paper provides images of the different types of isolated pores, particularly refining the discrimination of variants within the Cymbellales, and reveals some interesting ecological patterns.
View Article and Find Full Text PDFSTAR Protoc
December 2024
Nantes Université, CNRS, US2B, UMR 6286, 44000 Nantes, France; Institute for Marine and Antarctic Studies (IMAS), Ecology and Biodiversity Centre, University of Tasmania, Hobart, TAS 7004, Australia. Electronic address:
Meticulous sample preparation and strict adherence to preservation procedures are essential for electron microscopy investigations, which enable accurate capture of organisms' morphology, size, and potential interactions within the sample. Here, we present a protocol for preserving cells of the model diatom Phaeodactylum tricornutum and its native bacterial community. We describe steps for diatom fixation and coverslip preparation and washing.
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