Aim: To identify if any difference exisit between the population of Sichuan and Qinling giant panda. express IL-2 protein in BL21(DE3)with biological activity.
Methods: IL-2 was amplified by RT-PCR from adult Qinling giant panda peripheral blood lymphocyte which was induced by ConA, and cloned into prokaryotic expression vector of pET32a-IL-2. The fusion protein was expressed in BL21(DE3)/pET system and identified by SDS-PAGE and Western blot; inoculate rabbit with purified expression products to prepare polyclonal antibody; use lymphocytes proliferation in vitro to detect biological activity.
Results: The protein of IL-2 was obtained by recombination expression, molecule weight is 34 000 .The specificity of polyclonal antibody was obtained, in vitro activity test indicated that the recombinant protein IL-2 having an activity of promoting the proliferation of lymphocytes. The effect can be stopped by polyclonal antibody which was prepared before.
Conclusion: The IL-2 gene of Qinling giant panda was cloned and expressed in E.coli successfully, and the homology of IL-2 gene in these two population is 99.4% and the recombination protein can promote the proliferation of lymphocytes in vitro from giant panda.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Ecological and anthropogenic drivers of local extinction and colonization of giant pandas over the past 30 years.
Ecology
January 2025
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, China.
Understanding the patterns and drivers of species range shifts is essential to disentangle mechanisms driving species' responses to global change. Here, we quantified local extinction and colonization dynamics of giant pandas (Ailuropoda melanoleuca) using occurrence data collected by harnessing the labor of >1000 workers and >60,000 worker days for each of the three periods (TP1: 1985-1988, TP2: 1998-2002, and TP3: 2011-2014), and evaluated how these patterns were associated with (1) protected area, (2) local rarity/abundance, and (3) abiotic factors (i.e.
View Article and Find Full Text PDFNon-Structural Protein V of Canine Distemper Virus Induces Autophagy via PI3K/AKT/mTOR Pathway to Facilitate Viral Replication.
Int J Mol Sci
December 2024
Key Laboratory of Veterinary Medicine in Universities of Sichuan Province, College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, 16 Yihuan Rd., Chengdu 610041, China.
Canine distemper (CD) is a highly infectious disease of dogs which is caused by canine distemper virus (CDV). Previous studies have demonstrated that CDV infection can induce autophagy in cells. However, the mechanism underlying CDV-induced autophagy remains not fully understood.
View Article and Find Full Text PDFLow Reproductivity of Giant Pandas May Be Associated with Increased Vaginal .
Microorganisms
December 2024
Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China.
The poor reproductive capacity of giant pandas significantly hinders the development of captive populations, with 80.88% of adult individuals being unable to successfully become pregnant and deliver offspring. The disturbance of vaginal microbiota has been proven to potentially lead to miscarriage, abortion, and stillbirth in mammals.
View Article and Find Full Text PDFComplete Mitochondrial Genomes of and with Phylogenetic Analysis of Charadriiformes.
Genes (Basel)
December 2024
Shaanxi Key Laboratory of Qinling Ecological Security, Shaanxi Institute of Zoology, Xi'an 710032, China.
Background: Plovers (Charadriidae), within the order of Charadriiformes, a group of modern birds distributed worldwide, are a frequent subject of molecular phylogenetic studies. While research on mitochondrial genome (mitogenome) variation within the family Charadriidae, especially intraspecific variation, is limited. Additionally, the monophyly of and the phylogenetic placement of remain contentious.
View Article and Find Full Text PDFPredicting Body Weight from Birth to Old Age in Giant Pandas Using Machine Learning.
Animals (Basel)
December 2024
Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China.
The giant panda () is one of the animals with the largest body weight differences between its birth and adult stages, where the newborn cub is 0.1% the size of its mother. The rapid growth of panda cubs has been reported previously, but little is known about the growth pattern of their entire lifetime.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!