Hits and misses from gene expression ratio measurements in cDNA microarray studies.

J Biomol Tech

Department of Neuroscience, Molecular Biology Core, Kennedy Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Published: September 2002

DNA microarray users face many challenges to obtain accurate results, including complex technical errors, natural variability of biological systems, imperfect reproducibility of reference standards, and difficulties in acquisition and processing of large amounts of data. Therefore, investigators should be aware of potential sources of variability and account for them in the experimental design and execution. This work reports our experience in identifying factors that alter the reliability of the results and in diminishing effects of these factors. We have studied the hybridization reproducibility in cDNA microarray chips, both as absolute values and expression ratios, and the nature and impact of several technical, acquisition, and processing errors. A new experimental strategy is proposed and mathematical algorithms developed that efficiently correct the errors and thereby increase the information obtainable through microarray studies. These algorithms reduced the variability not associated with biological system to less than a quarter of its initial value and have substantially enhanced reliability in experiments on brain and cultured neuroblastoma cells.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2279857PMC

Publication Analysis

Top Keywords

cdna microarray
8
microarray studies
8
acquisition processing
8
hits misses
4
misses gene
4
gene expression
4
expression ratio
4
ratio measurements
4
measurements cdna
4
microarray
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!