The effect of optical superresolution on speckle correlations is studied. Simulations reveal that using a lateral superresolution pupil filter more than twice the out of plane correlation length of the clear pupil can be achieved. This means that the measurement range in speckle correlation measurements doubles. To verify the correlation length an experiment is performed using a liquid crystal (LCD) spatial light modulator as a programmable superresolution filter. The results corroborate the simulation.
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http://dx.doi.org/10.1364/opex.13.006468 | DOI Listing |
Anal Chem
December 2024
Guangdong Key Laboratory of Nanomedicine, CAS-HK Joint Lab of Biomaterials, CAS Key Laboratory of Biomedical Imaging Science and System, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology (SIAT), Chinese Academy of Sciences, Shenzhen 518055, P. R. China.
Lysosomes are acidic membrane-bound organelles that aid digestion, excretion, and cell renewal. The lysosomal membranes are essential for maintaining lysosomal functions and cellular homeostasis. In this work, we developed a molecular "NOR" logic gate, , by introducing malachite green into the spirocyclic rhodamine.
View Article and Find Full Text PDFLocalization microscopy enables imaging with resolutions that surpass the conventional optical diffraction limit. Notably, the Maximally INFormative LUminescence eXcitation (MINFLUX) method achieves super-resolution by shaping the excitation point spread function (PSF) to minimize the required photon flux for a given precision. Various beam shapes have recently been proposed to improve localization efficiency, yet their optimality remains an open question.
View Article and Find Full Text PDFAdv Sci (Weinh)
December 2024
Department of Bioengineering, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, Cancer Center at Illinois, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
High-resolution optical microscopy, particularly super-resolution localization microscopy, requires precise real-time drift correction to maintain constant focus at nanoscale precision during the prolonged data acquisition. Existing methods, such as fiducial marker tracking, reflection monitoring, and bright-field image correlation, each provide certain advantages but are limited in their broad applicability. In this work, a versatile and robust drift correction technique is presented for single-molecule localization-based super-resolution microscopy.
View Article and Find Full Text PDFNeural Netw
December 2024
College of Electronic and Information Engineering, Tongji University, China; Shanghai Institute of Intelligent Science and Technology, Tongji University, China. Electronic address:
The target of space-time video super-resolution (STVSR) is to increase both the frame rate (also referred to as the temporal resolution) and the spatial resolution of a given video. Recent approaches solve STVSR using end-to-end deep neural networks. A popular solution is to first increase the frame rate of the video; then perform feature refinement among different frame features; and at last, increase the spatial resolutions of these features.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
December 2024
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen 37077, Germany.
Optical imaging access to nanometer-level protein distributions in intact tissue is a highly sought-after goal, as it would provide visualization in physiologically relevant contexts. Under the unfavorable signal-to-background conditions of increased absorption and scattering of the excitation and fluorescence light in the complex tissue sample, superresolution fluorescence microscopy methods are severely challenged in attaining precise localization of molecules. We reasoned that the typical use of a confocal detection pinhole in MINFLUX nanoscopy, suppressing background and providing optical sectioning, should facilitate the detection and resolution of single fluorophores even amid scattering and optically challenging tissue environments.
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