AI Article Synopsis
- The study examined how H(2)S preconditioning affects heart protection in rats after a heart attack, showing it significantly reduces infarct size when administered a day prior.
- It was found that the protective effects of this preconditioning lasted for at least three days, while post-infarction H(2)S treatment was less effective.
- The research concluded that the protective mechanism relies on protein kinase C (PKC), highlighting that preconditioning is more beneficial than treatment after the heart attack, and combining both does not enhance protection.
Article Abstract
In the current study, we investigated the delayed cardioprotection induced by H(2)S preconditioning in an in vivo rat model of myocardial infarction. Assessment of infarct size revealed that a single bolus of NaHS (a donor of H(2)S, at 0.1-10 micromol/kg body weight) administered 1 day before myocardial infarction produced a strong infarct-limiting effect. A time course study demonstrated that the protection lasted at least 3 days after the preconditioning stimulus. We further compared the effect of H(2)S preconditioning with post-infarction treatment. Although injection of NaHS (1 micromol/kg once daily) for 3 days after myocardial infarction also significantly decreased infarct size, the protective effect was significantly lower than that afforded by H(2)S preconditioning. A combination of both preconditioning and post-treatment did not produce a stronger protection compared with H(2)S preconditioning alone. Pretreatment with chelerythrine chloride (5 mg/kg, i.p.), a protein kinase C (PKC) inhibitor, 15 min before NaHS administration blocked the infarct-sparing effect of H(2)S preconditioning. In conclusion, the current study provided the first evidence that H(2)S preconditioning produces strong late cardioprotection through a PKC-dependent mechanism. Such protection could not be reproduced by H(2)S treatment after the infarction occurred. A combination of both preconditioning and post-treatment does not provide additional benefit and hence is not necessary when the access to preconditioning has been secured.
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| http://dx.doi.org/10.1016/j.ejphar.2009.05.023 | DOI Listing |
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