AI Article Synopsis

  • BPA (Bisphenol A) is a chemical found in polycarbonate plastics, known for its weak estrogenic properties and its classification as an endocrine disruptor.
  • A specific anti-BPA antibody was created using a unique method, which showed minimal interference with similar compounds and no interaction with natural steroids.
  • The newly developed BPA radioimmunoassay (RIA) has reliable detection capabilities, demonstrating high recovery rates and a strong correlation with other testing methods, making it effective for assessing human BPA exposure.

Article Abstract

Bisphenol A (BPA) is widely used in the manufacturing of polycarbonate plastic food and drink packaging. Possessing a weak estrogenic activity, BPA is listed among a growing list of endocrine disrupting compounds. In this study, a polyclonal anti-BPA antibody was obtained by immunization with BPA-monocarboxymethylether covalently linked to BSA. The antibody demonstrates negligible cross-reactivity with most analogous BPA phenolic structures, and no cross-reactivity with endogenous steroids. An extraction step with ethyl acetate minimized matrix effects and allowed the BPA measurement in plasma and other biological samples. Recovery after loading test was 96 +/- 4% and dilution tests had a linear profile (r2 > 0.93). The limit of detection of the BPA RIA was 0.08 microg L(-1) with an IC50 of 1.25 microg L(-1). The intra- and inter-assay coefficients of variation were 5.6 and 8.6%, respectively at a BPA concentration of 0.7 microg L(-1) and 6.9 and 5.7% at a BPA concentration of 1.3 microg L(-1). A significant correlation was found between the values obtained by the RIA and HPLC-MS (r2 = 0.92) or HPLC coupled to a fluorescence detector (r2 = 0.80). In conclusion, we described a BPA-RIA that is a suitable tool for evaluating human exposure to BPA.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2009.04.036DOI Listing

Publication Analysis

Top Keywords

microg l-1
16
biological samples
8
bpa
8
bpa concentration
8
concentration microg
8
development radioimmunoassay
4
radioimmunoassay measurement
4
measurement bisphenol
4
bisphenol biological
4
samples bisphenol
4

Similar Publications

Semi-aerobic Aged-refuse Bioreactor (SAARB) has a good effect on nitrogen removal in leachate, but a strong greenhouse gas (N2O) was generated during the nitrification and denitrification process. The effect of salinity (7-30 g x L(-1)) on the leachate treatment and the N2O production from SAARB system was investigated. Experimental results showed that salinity ranging from 7 to 30 g x L(-1) had no significant effect on COD removal, and the removal efficiency was always more than 85%.

View Article and Find Full Text PDF

In order to investigate the feasibility of deep denitrification and simultaneous removing phthalate esters (PAEs) in the process of reclaimed water treatment uses three-dimensional biofilm-electrode reactor coupled with sulfur autotrophic deep denitrification technology (3BER-S), the technological characteristics and mechanisms were analyzed based on determining the static adsorption capacity of biofilm cultured active carbon fillers in 3BER-S reactor together with the operation results of dynamic denitrification and simultaneous PAEs removing. The results showed that the average adsorption rates of DBP, DEHP were 85.84% and 97.

View Article and Find Full Text PDF

An analysis method has been established to test 27 elements (Li, Be, B, Mg, Al, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Sr, Mo, Cd, Sn, Sb, Ba, La, Hg, Pb, Bi) in Maca nationality's medicine with microwave digestion-ICP-MS. Sample solutions were analyzed by ICP-MS after microwave digestion, and the contents of elements were calculated according to their calibration curves, and internal standard method was adopted to reduce matrix effect and other interference effects. The experimental results showed that the linear relations of all the elements were very good; the correlation coefficient (r) was 0.

View Article and Find Full Text PDF

Based on indirect competitive immunoassay mechanism, bisphenol A (BPA) was detected by the evanescent wave all-fiber immunosensor previously developed with the detection limit of 0.2 microg x L(-1) and the linear detection range of 0.3-33.

View Article and Find Full Text PDF

In this paper, a method of determination of trace lead in water by UV-Visible diffuse reflectance spectroscopy combined with surfactant and membrane filtration enrichment was proposed. In the NH3 x H2O-NH4Cl buffer solution with pH 8.5, the lead(II) ion would react with dithizone to form the red complex under vigorous stirring, which is hydrophobic and can be enriched by the mixed cellulose ester membrane.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!