Background: Optic nerve regeneration has previously been achieved by injuring the lens, which results in the release of lentogenic factors. However, these lentogenic factors are still unknown.
Objectives: To investigate what were the lentogenic factors by examining the effects of lens extract and macrophage-conditioned medium (MCM) on the survival and the neurite outgrowth of rat retinal neurons in vitro.
Methods: Retinal neurons were cultured in 4 groups: (1) Dulbecco's modified Eagle's medium (DMEM), (2) DMEM containing lens extract, (3) DMEM containing macrophage-conditioned medium (MCM-D), (4) DMEM and medium from macrophages grown with lens extract (MCM-L). Neurite outgrowth and neuron survival time were observed. The density of retinal neurons with neurites and the longest neurites of the cells were measured on days 1, 3 and 5.
Results: Retinal neurons survive for 12-14 days in DMEM containing lens extract. However, the cells only survive for 6 days in DMEM and only 7 days in DMEM containing MCM-L or MCM-D. The present results indicate that lens extract may directly promote survival of rat retinal neurons and neurite outgrowth in vitro. The MCM also promoted cell survival and neurite outgrowth but its effects were weaker than that of the lens extract. We postulate that lens extract exerts its effect by direct neurotrophic effects and/or indirectly by activating macrophages in vitro.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1159/000219682 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mechanical Properties of Eye Lens Cortical and Nuclear Membranes and the Whole Lens.
Invest Ophthalmol Vis Sci
January 2025
Department of Physics, Boise State University, Boise, Idaho, United States.
Purpose: To elucidate the mechanical properties of the bovine lens cortical membrane (CM), the nuclear membrane (NM) containing cholesterol bilayer domains (CBDs), and whole bovine lenses.
Methods: The total lipids (lipids plus cholesterol) from the cortex and nucleus of a single bovine lens were isolated using the monophasic methanol extraction method. Supported CMs and NMs were prepared from total lipids extracted from the cortex and nucleus, respectively, using a rapid solvent exchange method and probe-tip sonication, followed by the fusion of unilamellar vesicles on a flat, freshly cleaved mica surface.
Lucia Keratoprosthesis in a Sclerocorneal Graft for Recurrent Graft Rejection Secondary to Herpetic Keratitis.
Cornea
January 2025
Instituto de Oftalmologia Fundacion Conde de Valenciana IAP, Mexico City, Mexico.
Purpose: To report the surgical approach of a Lucia keratoprosthesis (KPro) on a sclerocorneal graft in the setting of recurrent graft rejection and perilimbal scleral thinning.
Methods: A case report.
Results: We report the case of a 26-year-old man with a history of herpes simplex keratitis, 2 penetrating keratoplasties, graft failure, secondary glaucoma, and a conjunctival flap in the right eye who underwent a Lucia KPro, lens extraction, glaucoma drainage device, and pars plana vitrectomy.
Brain tumour histopathology through the lens of deep learning: A systematic review.
Comput Biol Med
January 2025
Auckland Bioengineering Institute, The University of Auckland, New Zealand. Electronic address:
Problem: Machine learning (ML)/Deep learning (DL) techniques have been evolving to solve more complex diseases, but it has been used relatively little in Glioblastoma (GBM) histopathological studies, which could benefit greatly due to the disease's complex pathogenesis.
Aim: Conduct a systematic review to investigate how ML/DL techniques have influenced the progression of brain tumour histopathological research, particularly in GBM.
Methods: 54 eligible studies were collected from the PubMed and ScienceDirect databases, and their information about the types of brain tumour/s used, types of -omics data used with histopathological data, origins of the data, types of ML/DL and its training and evaluation methodologies, and the ML/DL task it was set to perform in the study were extracted to inform us of trends in GBM-related ML/DL-based research.
Acidogenic fermentation of Ulva in a fed-batch reactor system: tubular versus foliose biomass.
Enzyme Microb Technol
December 2024
University of Galway, University Road, Galway H91 TK33, Ireland; Science Foundation Ireland MaREI Centre for Energy, Climate and Marine, Environmental Research Institute, University College Cork, Cork, Ireland.
The present study proposes a biorefinery of the macroalgae Ulva, focusing on evaluating two different morphologies of the species (foliose and tubular) during acidogenic fermentation in fed-batch reactors. Stage 1 of the study evaluates lyophilised foliose and tubular Ulva, whilst Stage 2 analyses the impact of ulvan extraction on volatile fatty acids yield and changes in carbohydrate availability. Acetic, propionic, and butyric acids were produced from each substrate, with peak concentrations of total VFAs recorded at 2179.
View Article and Find Full Text PDFExplanatory models of common mental disorders among South Asians in high-income countries: A systematic review.
Transcult Psychiatry
January 2025
Department of Population Health, London School of Hygiene and Tropical Medicine, London, UK.
Mental health service use by individuals of South Asian origin living outside of South Asia is influenced by cultural factors such as endorsing psycho-social-spiritual over biological explanations, somatisation, and stigma. The aim of this review is to synthesise the evidence about (a) explanatory models of common mental disorders (CMDs) among people of South Asian origin residing in high-income countries, and (b) their help-seeking for CMDs, including formal and informal care. The systematic review protocol was registered a priori on Prospero (registration number CRD42021287583).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!