AI Article Synopsis
- The TZM-bl cell line, used for testing neutralizing antibodies against HIV-1, has been found to be contaminated with murine leukemia virus (MLV), which raises concerns about previous research findings.
- Researchers confirmed this contamination and tested HIV-1 neutralization in a different cell line that doesn’t have MLV.
- Results showed that the neutralization assays were consistent between the contaminated TZM-bl cells and the clean cell line, suggesting the MLV contamination does not impact the effectiveness of HIV-1 neutralization results.
Article Abstract
The TZM-bl cell line that is commonly used to assess neutralizing antibodies against human immunodeficiency virus type 1 (HIV-1) was recently reported to be contaminated with an ecotropic murine leukemia virus (MLV) (Y. Takeuchi, M. O. McClure, and M. Pizzato, J. Virol. 82:12585-12588, 2008), raising questions about the validity of results obtained with this cell line. Here we confirm this observation and show that HIV-1 neutralization assays performed with a variety of serologic reagents in a similar cell line that does not harbor MLV yield results that are equivalent to those obtained in TZM-bl cells. We conclude that MLV contamination has no measurable effect on HIV-1 neutralization when TZM-bl cells are used as targets for infection.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715758 | PMC |
| http://dx.doi.org/10.1128/JVI.00709-09 | DOI Listing |
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