Objectives: Recently, a CTX-M-15 extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone.
Methods: A total of 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene.
Results: One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris.
Conclusions: We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/jac/dkp194 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Investigation of Virulence Factors, Phylogenetic Group Features, and the Presence of ST131 Clone in Escherichia coli Isolates, a Urinary Tract Infection Agent in Children].
Mikrobiyol Bul
October 2023
Ankara University Faculty of Medicine, Department of Medical Microbiology, Ankara, Türkiye.
Article Synopsis
- Urinary tract infections (UTIs) in children caused by E.coli, especially the O25b/ST131 strain, are concerning due to their increased virulence and resistance to antibiotics.
- A study analyzed 200 E.coli isolates from UTI cases in pediatric patients to assess their antimicrobial resistance, virulence factors, and phylogenetic groupings, aiming to improve UTI management strategies.
- The results revealed high resistance rates to commonly used antibiotics, highlighting the need for better diagnostic and treatment approaches for UTI in children.
Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital.
Microorganisms
August 2023
Centro Médico ISSEMYM Toluca, Av. Baja Velocidad KM. 57.5, Carr. Mex./Tol. Col. San Jeronimo Chicahualco, Metepec 52170, Edomex, Mexico.
Article Synopsis
- - The study investigated the prevalence of plasmid-mediated colistin resistance genes among antibiotic-resistant Gram-negative bacteria in a hospital in Toluca, Mexico, using PCR to analyze 241 collected strains.
- - Out of these strains, 12 (5.0%) were found to be colistin-resistant, with two isolates carrying the mcr-1 gene and belonging to the O25b-ST131 clone, known for its ability to transmit resistance.
- - The findings highlight the alarming presence of colistin resistance in hospitalized patients in Mexico and indicate that the resistant O25b-ST131 clone can acquire and spread resistance genes among various bacteria.
[Investigation of O25b-ST131 Clone Frequency and Presence of Carbapenem and Fosfomycin Resistance Genes in Escherichia coli Isolates: First Detection of fosA3 from Escherichia coli O25bSTf131 Clone from Türkiye].
Mikrobiyol Bul
July 2023
Ondokuz Mayıs University Faculty of Medicine, Department of Medical Microbiology, Samsun, Türkiye.
Article Synopsis
- The Escherichia coli ST131 clone, identified as a 'high-risk pandemic clone' since 2008, significantly contributes to the global spread of antimicrobial resistance and carries genes for both virulence and antibiotic resistance.
- A study investigated the prevalence of the O25b-ST131 clone and the presence of antibiotic resistance genes in fluoroquinolone-resistant E.coli isolates from urine samples, using specific PCR methods for detection.
- Results showed that 51.2% of isolates were identified as E.coli O25b-ST131, with carbapenem and fosfomycin resistance rates at 3.41%, and specific resistance genes detected in some isolates, highlighting the concerning rise in antibiotic resistance.
Investigation of the presence of Capnophilic bacteria in routine urine cultures.
Eur J Clin Microbiol Infect Dis
April 2023
Department of Medical Microbiology, Ankara University School of Medicine, Ankara, Turkey.
Article Synopsis
- - The study focused on the rare Capnophilic Escherichia coli (CEC) strains found in urinary tract infections (UTIs), analyzing their incidence and features in patients.
- - Out of 8500 urine samples, only nine CEC isolates (0.11%) were identified, with three belonging to a specific clone (O25b-ST131) and displaying varying antibiotic resistance.
- - The researchers noted that isolating CEC strains is challenging due to their specific growth requirements and suggested that capnophilic incubation could be beneficial for patients with certain health conditions.
Epidemiology of extended-spectrum β-lactamases in in Taiwan for over two decades.
Front Microbiol
January 2023
Department of Intensive Care Medicine, Chi Mei Medical Center, Tainan, Taiwan.
Article Synopsis
- Antimicrobial resistance, particularly regarding ESBL-producing bacteria, is a significant public health issue, with variations in prevalence noted across different regions, species, and settings in Taiwan.
- The study found higher rates of ESBL producers in northern Taiwan, with SHV-type and CTX-M-type ESBLs being the most common, particularly linked to certain bacterial species.
- A concerning clone, O25b-ST131, has emerged in urinary and bloodstream infections, showing virulence and resistance, underscoring the need for ongoing surveillance and research on ESBL in Taiwan.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!