AI Article Synopsis
- Developed two genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura to allow easy modification of large genomic clones.
- The fosmid transgenes created mimic natural gene expression patterns in these flies.
- This technology will help in rescuing mutant traits, visualizing gene products in live flies, and studying gene activity across different species.
Article Abstract
We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.
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| http://dx.doi.org/10.1038/nmeth.1334 | DOI Listing |
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