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Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants. | LitMetric
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Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants.

Maria Jönsson Zhian Saleihan Ingolf F Nes Helge Holo

Appl Environ Microbiol

Department of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, N-1432 As, Norway.

Published: July 2009

The roles of the two ldh genes of Enterococcus faecalis were studied using knockout mutants. Deletion of ldh-1 causes a metabolic shift from homolactic fermentation to ethanol, formate, and acetoin production, with a high level of formate production even under aerobic conditions. Ldh-2 plays only a minor role in lactate production.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2708445PMC
http://dx.doi.org/10.1128/AEM.00344-09DOI Listing

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