A sensitive ultra performance liquid chromatography-mass spectrometry method has been developed and validated for the quantification of taxifolin in rat plasma. Following liquid/liquid extraction by ethyl acetate, the analytes were separated on a Sunfire (2.1 mm x 50 mm, 3.5 microm) column and analyzed in the selected ion recording with a negative electrospray ionization mode. The method was linear over the concentration range of 6-6750 ng/mL. Intra- and inter-day precisions were all within 8% and accuracy ranged from 92.9% to 105.1%. The lower limit of quantification was 6 ng/mL. The present method was successfully applied to the estimation of the pharmacokinetic parameters of taxifolin following intravenous and oral administration to rats. The absolute bioavailability of taxifolin was 0.17% in rat.
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| http://dx.doi.org/10.1016/j.jchromb.2009.04.037 | DOI Listing |
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