[Gas chromatography-mass spectrometric analysis of Jatropha curcas leaf extracts prepared by supercritical fluid CO2 extraction].

Nan Fang Yi Ke Da Xue Xue Bao

School of Life Science and Biopharmacology, Guangdong Pharmaceutical University, and Department of Pharmaceutics, Zhujiang Hospital, Guangzhou 510006, China.

Published: May 2009

Objective: To analyze the bioactive components in Jatropha curcas leaves using gas chromatography-mass spectrometry (GC-MS).

Methods: The bioactive components were extracted from J. curcas leaves by supercritical fluid CO2 extraction and analyzed by using GC-MS.

Results: Seventy peaks were detected by GC-MS, and 43 compounds were identified (61.43%). Among the identified compounds, 16 had a content of more than 1%, and the total contents of these 16 compounds reached 81.36%. The four most abundant components were 22,23-dihydro-stigmasterol (16.14%), alpha-tocopherol (15.18%), beta-amylin (7.73%) and dotriacontanol (7.02%). The content of gamma-tocopherol reached 2.88% and vitamin E reached 18.06% in the extract.

Conclusion: J. curcas leaves contain multiple compounds with anti-tumor, anti-virus and antimicrobial activities.

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