AI Article Synopsis
- The study tested two primer pairs (ITS86F/ITS4 and ITS1-F/ITS86R) to amplify DNA from soil fungal communities.
- The results showed that the ITS86F/ITS4 pair was specific for major fungal groups (Ascomycetes, Basidiomycetes, Zygomycetes), while ITS1-F/ITS86R amplified Ascomycetes and Basidiomycetes but not Zygomycetes.
- This research is the first to evaluate these primer pairs for effectively analyzing fungal DNA in soil environments.
Article Abstract
Internal transcribed spacer (ITS) 86F and ITS4 and the ITS1-F and ITS86R primer pairs were tested to specifically amplify fungal community DNA extracted from soil. Libraries were constructed from PCR-amplified fragments, sequenced and compared against sequences deposited in GenBank. The results confirmed that the ITS86F and ITS4 primer pair was selectively specific for the Ascomycetes, Basidiomycetes and Zygomycetes fungal clades. Amplified products generated by the ITS1F and ITS86R primer pair also aligned with sequences from a range of species within the Ascomycete and Basidiomycete clades but not from the Zygomycete. Both primer sets demonstrated fungal specificity and appear to be well suited for rapid PCR-based (fingerprinting) analysis of environmental fungal community DNA. This is the first reported use and assessment of the ITS86F and ITS4 and the ITS1-F and ITS86R primer pairs in amplifying fungal community DNA from soil.
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| http://dx.doi.org/10.1111/j.1574-6968.2009.01621.x | DOI Listing |
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