Improving the catalytic performance of fungal laccases in monoterpene-based reaction systems.

Biotechnol Lett

Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece.

Published: September 2009

Ternary systems consisting of monoterpenes (alpha-pinene or D-limonene), tert-butanol and water were used as reaction media to enhance the catalytic performance of laccases from various fungi sources (Trametes versicolor, T. hirsuta and Botrytis cinerea). The enzymes had improved catalytic efficiency (5- to 10-fold) in alpha-pinene-rich environment, while optimal reaction rates were in high-water content systems (15.5% v/v). The stability of laccases was significantly improved in monoterpene-based systems (up to 90% residual enzyme activity after 24 h at 30 degrees C) in comparison with other non-conventional media. The results indicate that these ternary systems can increase the potential of laccases as catalysts for various oxidations.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-009-0014-5DOI Listing

Publication Analysis

Top Keywords

catalytic performance
8
ternary systems
8
systems
5
improving catalytic
4
performance fungal
4
laccases
4
fungal laccases
4
laccases monoterpene-based
4
monoterpene-based reaction
4
reaction systems
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!