Samples were digested by microwave digestion. The selenium content in selenium enriched Cordyceps militaris was determined by ICP-MS method, HPLC/fluorometric method, and 3,3-diaminobenzidine method separately. And the detection conditions, the lowest detection limit and the relative standard deviation (RSD) of the three determination methods were compared. The detection conditions of the three methods for the detection of selenium content in selenium enriched Cordyceps militaris were established. It was showed that the lowest detection limit of ICP-MS method, HPLC/fluorometric method, and 3,3-diaminobenzidine method was 0.260 7, 0.182 1 and 10.485 9 microg x L(-1) respectively, and this means that the lowest detection limit of ICP-MS method was the lowest and that of 3,3-diaminobenzidine method was the highest. For the same sample the relative standard deviation (RSD) of ICP-MS method was the lowest and the RSD of 3,3-diaminobenzidine method was the highest. It was recommended that selenium content is determined by ICP-MS and HPLC/fluorometric method when the selenium in the sample is very low and by 3,3-diaminobenzidine method when the content is rather high.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Simple and rapid plaque assay for recombinant baculoviruses expressing influenza hemagglutinin.
Sci Prog
April 2022
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Republic of Korea.
Recombinant baculoviruses (rBVs) have been extensively used to generate virus-like particles, and baculoviruses expressing antigenic proteins have become efficient tools for inducing protective immunity. However, current methods for generating baculoviruses are costly and inefficient. Thus, the development of a simple, rapid, and accurate method of baculovirus titration is critically important.
View Article and Find Full Text PDFInsights into soy lecithin and egg yolk-based extenders for chilling canine spermatozoa.
Zygote
February 2019
University of São Paulo,College of Veterinary Medicine and Animal Science,Department of Animal Reproduction, Cidade Universitária, São Paulo,Brazil.
SummaryThe aim of this study was to compare different concentrations of soy lecithin (LEC0.01%, LEC0.05% and LEC0.
View Article and Find Full Text PDFBacillus amyloliquefaciens alters gene expression, ROS production and lignin synthesis in cotton seedling roots.
J Appl Microbiol
June 2018
Department of Plant Biology, Rutgers University, New Brunswick, NJ, USA.
Aims: Previous research demonstrated that applying Bacillus amyloliquefaciens to cotton seeds promotes growth, alters root architecture and alleviates salt stress of cotton seedlings. This research was undertaken to further study the genetic responses elicited in cotton seedlings by this growth promoting bacterium.
Methods And Results: GeneChip microarrays and RT-qPCR were used to detect changes in gene expression in seedling roots inoculated with B.
Effects of Soy Lecithin Extender on Dog Sperm Cryopreservation.
Anim Biotechnol
July 2018
a University of São Paulo, College of Veterinary Medicine and Animal Science, Department of Animal Reproduction , São Paulo , Brazil.
Semen cryopreservation is an essential biotechnology in canine reproduction and during the cryopreservation process commonly egg yolk are used. The discrepancy in the egg yolk composition and the potential risk of disease dissemination are obstacles for semen exportation and use. Therefore, studies aiming to substitute egg yolk are extremely important.
View Article and Find Full Text PDFDemonstration of epithelial-mesenchymal transition in kidney. The contribution of a coupled histochemical and immunohistochemical staining with Periodic Acid-thionin Schiff.
Appl Immunohistochem Mol Morphol
March 2008
Federal University of Espirito Santo School of Medicine, Santo, Brazil.
Traditional Periodic Acid Schiff has been extensively used, coupled with immunohistochemistry for epithelia or mesenchymal cells, to highlight renal tubular basement membrane (TBM). We recently tried to perform such technique in a 5/6 nephrectomy model of progressive renal fibrosis to demonstrate TBM disruption as an evidence for epithelial-mesenchymal transdifferentiation. Despite excellent basement membrane staining with traditional fuchsin-Periodic Acid Schiff, the interface between epithelial and mesenchymal cells was frequently blurred when revealed with 3'3 diaminobenzidine tetrachloride-peroxidase.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!