We modified the conventional PFGE procedure for Serratia marcescens to establish a rapid method. Our protocol showed modification in the bacterial lysis, washing, and restriction enzyme digestion time. This resulted in shortening the time needed by about 3 days compared to the conventional PFGE method.
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http://dx.doi.org/10.1016/j.mimet.2009.05.007 | DOI Listing |
Antibiotics (Basel)
November 2024
Department of Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn Str. 8-10, D-10589 Berlin, Germany.
The increasing occurrence of extended-spectrum β-lactamase (ESBL)-producing , most commonly , has become a serious problem. The aim of this study was to determine the presence of ESBL-producing Gram-negative bacteria in dairy cattle, goat and sheep farms located in southern Türkiye. Samples (409 quarter milk samples and 110 fresh faecal samples from cattle, 75 bulk tank milk samples and 225 rectal swab samples from goats and sheep) were subjected to selective isolation on MacConkey agar with ceftazidime (2 µg/mL).
View Article and Find Full Text PDFJ Infect Public Health
December 2024
Department of Medical Microbiology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey; Department of Medical Biotechnology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey. Electronic address:
Background: Drug-resistant Group A beta-hemolytic streptococci remain significant infectious agents globally. This study investigated the major S. pyogenes strains responsible for infections in Türkiye and their susceptibility to beta-lactam and macrolide antibiotics.
View Article and Find Full Text PDFWei Sheng Yan Jiu
November 2024
Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China.
Objective: To investigate the molecular typing characteristics, drug resistance status and drug resistance gene carrying of food-borne Staphylococcus aureus in Ningxia.
Methods: Staphylococcus aureus isolated from food safety risk monitoring project in Ningxia in the past ten years were collected, drug resistance was detected using microbroth dilution method, enterotoxins were detected by real-time PCR. The strains were genotyped by pulsed field gel electrophoresis(PFGE) using SmaI endonuclease.
Eur J Clin Microbiol Infect Dis
December 2024
Service de Bactériologie et des Contrôles Microbiologiques, CHU Nantes, Nantes, France.
Purpose: Pseudomonas aeruginosa clinical strains isolated harbored sometimes an atypical phenotype using the automated Vitek2: ciprofloxacin-susceptibility but levofloxacin-resistance according to 2019 CA-SFM criteria. The aims of this study are to investigate the resistance mechanism(s) involved and to identify the consequences on fluoroquinolone treatment.
Methods: Strain resistance profile, patient's data were recovered and reviewed from the database.
J Glob Antimicrob Resist
December 2024
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China; Xianghu Laboratory, Hangzhou, China. Electronic address:
Objectives: Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment.
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