AI Article Synopsis
- The study focuses on the triterpene product profile of At5g36150 (PEN3) in Arabidopsis thaliana, revealing that it produces primarily tirucalla-7,24-dien-3beta-ol, with minor products.
- It highlights the unexpected convergent evolution of another synthase, LUP5, and examines the molecular mechanisms behind the 20S configuration and adaptations in the active site for the 17alpha side chain.
- This research completes the functional characterization of all triterpene synthases in higher plants for the first time, marking a significant milestone in plant biochemistry.
Article Abstract
The triterpene product profile is reported for At5g36150 (PEN3), the last unexamined oxidosqualene cyclase in the reference plant Arabidopsis thaliana. PEN3 makes tirucalla-7,24-dien-3beta-ol ( approximately 85%) and several minor products. Also discussed are the unexpectedly facile convergent evolution of another Arabidopsis tirucalladienol synthase (LUP5), mechanistic origins of the 20S configuration, and active-site remodeling necessary to accommodate the 17alpha side chain. This work marks the first completed functional characterization of all triterpene synthases in a higher plant.
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| http://dx.doi.org/10.1021/ol9005745 | DOI Listing |
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