A simple and sensitive fluorescence quenching method was developed for the examination of cefadroxil and cephradine. The method is based on the reaction of fluorescein with cefadroxil or cephradine, resulting in the formation of complexes with weak fluorescence, i.e. quenching the fluorescence intensity of the system. By the use of absorption spectroscopy, infrared spectroscopy and quantum chemistry calculation the reaction process of the cephalosporins and the bonding of reaction mechanism were basically analyzed. Based on this, the fluorescence spectrometry method for the determination of the contents of cefadroxil and cephradine was developed: The maximum excitation and emission wavelength was at 483 nm, and 517 nm and 519 nm respectively. The linear calibration curves were obtained over the cefadroxil and cephradine concentration ranges of 0.3-13.5 mg x L(-1) and 0.1-1.2 mg x L(-1) respectively. The proposed method has been successfully applied to the examination of the subject drugs in its pharmaceutical dosage with good precision and accuracy. The recoveries are 99.63%-99.91% and 99.71%-100.08% respectively.
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J Appl Microbiol
October 2024
School of Pharmacy, The University of Sydney, 2006 NSW, Australia.
J Vet Sci
May 2024
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Importance: and contribute to global health challenges by forming biofilms, a key virulence element implicated in the pathogenesis of several infections.
Objective: The study examined the efficacy of various generations of cephalosporins against biofilms developed by pathogenic and .
Methods: The development of biofilms by both bacteria was assessed using petri-plate and microplate methods.
J Sep Sci
November 2022
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt.
Antimicrobial agents are essential to protect human and animal health. During the coronavirus disease 2019 pandemic, antimicrobials such as cephalosporins were widely used as prophylactics and to prevent bacterial co-infection. Undoubtedly, the prevalence of antibiotics in the aquatic environment will ultimately affect the degree of resistance against these bacteria in animals and the environmental systems.
View Article and Find Full Text PDFBiomed Res Int
August 2022
Department of Chemistry, Mirpur University of Science and Technology (MUST), Mirpur, 10250 AJK, Pakistan.
In present study, eleven cephalosporin drugs were selected to explore their new medically important enzyme targets with inherited safety advantage. To this end, selected drugs with active ingredient, cefpodoxime proxetil, ceftazidime, cefepime, ceftriaxone sodium, cefaclor, cefotaxime sodium, cefixime trihydrate, cephalexin, cefadroxil, cephradine, and cefuroxime, were evaluated and found to have significant activity against urease (IC50 = 0.06 ± 0.
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