Objective: To research the chemical constituents from Eleutherine americana.

Methods: Column chromatography with silica gel was employed to isolate and purify the constituents. Their structures were elucidated by means of MS and NMR.

Results: Eight constituents were obtained and identified as Isoeleutherol (1), eleutherin (2), isoeleutherin (3), beta-sitosterol(4), 8-hydroxy-3, 4-Dimethoxy-1-methyl-anthra-9, l0-quinone-2-carboxylic acid methyl ester (5), Hongconin (6), 4,8-Dihydroxy-3-Methoxy-1-methyl-anthra-9,10-quinone-2-carboxylic acid methyl ester (7), Eleutherinone (8), Kadsuric acid (9).

Conclusion: Compound 9 is isolated from the plant for the first time.

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