Contagious bovine pleuropneumonia (CBPP) caused by Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) has been eradicated in the developed world, but it is still present in many countries of sub-Saharan Africa. After initially successful control measures in the 1960s it has been spreading due to a lack of money, fragmentation of veterinary services, uncontrolled cattle movement, insufficient vaccine efficacy and sensitivity of current diagnostic tests. In this study we used two-dimensional polyacrylamide gel electrophoresis followed by immunoblot with sera from MmmSC-infected animals and MALDI-ToF mass spectrometry to identify novel immunogenic proteins as candidate molecules for improved diagnostics and vaccines. We identified 24 immunogens recognized by pooled sera from experimentally infected cattle. Furthermore, a serum from an animal with acute clinical disease as well as severe pathomorphological lesions recognized 13 additional immunogens indicating variation in the antibody responses to CBPP amongst cattle. Most immunogens showed compelling similarity to protein/gene sequences in the two ruminant pathogens M. capricolum subsp. capricolum and M. mycoides subsp. mycoides large colony type both belonging to the mycoides cluster. Three of these proteins, namely glycerol-3-phosphate oxidase, adenylosuccinate synthase, and glyceraldehyde-3-phosphate dehydrogenase, had no compelling homologue in the other distantly related bovine pathogen M. agalactiae. In addition, translation elongation factor Tu, heat shock protein 70, pyruvate dehydrogenase, and FKBP-type peptidyl-prolyl isomerase, which have been found to mediate adhesion to host tissue in other mycoplasmas were shown to be expressed and recognized by sera. These proteins have potential for the development of improved diagnostic tests and possibly vaccines.
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http://dx.doi.org/10.1016/j.vetimm.2009.04.016 | DOI Listing |
Heliyon
December 2024
Goat Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, 1341, Bangladesh.
Background And Objective: Contagious caprine pleuropneumonia (CCPP) is a highly contagious mycoplasmal respiratory disease primarily affecting goats and sheep caused by subsp. (Mccp). So far, there is no available information on either the serological or molecular identification of Mccp in Bangladesh.
View Article and Find Full Text PDFVet Anim Sci
December 2024
Department of Veterinary Epidemiology and Public Health, College of Veterinary Medicine and Animal Sciences, University of Gondar, Ethiopia.
Front Vet Sci
September 2024
Department of Veterinary Sciences, University of Turin, Turin, Italy.
Animals (Basel)
July 2024
Hainan Key Lab of Tropical Animal Reproduction, Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
subsp. (Mcc), a member of the cluster, has a negative impact on the goat-breeding industry. However, little is known about the pathogenic mechanism of Mcc.
View Article and Find Full Text PDFPLoS One
June 2024
ASTRE, CIRAD, INRAE, Univ Montpellier, Montpellier, France.
We investigated the interactions of unopsonized and opsonized Mycoplasma mycoides subsp. mycoides (Mmm) with bovine macrophages in vitro. Mmm survived and proliferated extracellularly on bovine macrophage cell layers in the absence of Mmm-specific antisera.
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