Protein cages have been used both as size-constrained reaction vessels for nanomaterials synthesis and as nanoscale building blocks for higher order nanostructures. We generated Janus-like protein cages, which are dual functionalized with a fluorescent and an affinity label, and demonstrated control over both the stoichiometry and spatial distribution of the functional groups. The capability to toposelectively functionalize protein cages has allowed us to manipulate hierarchical assembly using the layer-by-layer assembly process. Janus-like protein cages expand the toolkit of nanoplatforms that can be used for directed assembly of nanostructured materials.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/nl9009028 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fluorescein-based SynNotch adaptors for regulating gene expression responses to diverse extracellular and matrix-based cues.
Nat Commun
January 2025
Department of Biomedical Engineering, Boston University, Boston, MA, USA.
Synthetic Notch (SynNotch) receptors function like natural Notch proteins and can be used to install customized sense-and-respond capabilities into mammalian cells. Here, we introduce an adaptor-based strategy for regulating SynNotch activity via fluorescein isomers and analogs. Using an optimized fluorescein-binding SynNotch receptor, we describe ways to chemically control SynNotch signaling, including an approach based on a bio-orthogonal chemical ligation and a spatially controllable strategy via the photo-patterned uncaging of an o-nitrobenzyl-caged fluorescein conjugate.
View Article and Find Full Text PDFAlthough the toxic effect of Sedentary behavior (SED) on bone health has been demonstrated in the previous study, the underlying mechanisms of SED, or break SED to bone health remain unclear. In this study, we aim to investigate the effects of sedentary behavior (SED) on bone health, as well as the potential favor effects of moderate to vigorous physical activity (MVPA) and periodic interruptions of SED. To simulate SED, we used small Plexiglas cages (20.
View Article and Find Full Text PDFEffects of stocking density on growth performance, digestive enzyme activity, biochemical composition, and economic efficiency of a new strain of reared in cage culture system at Sindh Pakistan.
Food Sci Nutr
January 2025
Environmental and Life Sciences Programme, Faculty of Science Universiti Brunei Darussalam Jalan Tungku Link Gadong Brunei Darussalam.
The latest strain of is an altered ecological adaptation for sustainable aquaculture and is necessary to sustain stocking density and reduce physiological stress of the new strain. The present study aimed to determine the optimum stocking density, biological performance, and economic efficiency of the Nile tilapia. The 14,000 healthy seeds and uniform weight (40 ± 2.
View Article and Find Full Text PDFβ-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1.
BMC Immunol
January 2025
Laboratory of Oncology, Medical Research Center, The Second People's Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical University, Changzhou, China.
Background: B lymphocytes, essential in cellular immunity as antigen-presenting cells and in humoral immunity as major effector cells, play a crucial role in the antitumor response. Our previous work has shown β-glucan enhanced immunoglobulins (Ig) secretion. But the specific mechanisms of B-cell activation with β-glucan are poorly understood.
View Article and Find Full Text PDFThe Balbiani body is formed by microtubule-controlled molecular condensation of Buc in early oogenesis.
Curr Biol
January 2025
Department of Developmental Biology and Cancer Research, The Hebrew University of Jerusalem Faculty of Medicine, Ein-Kerem Campus, Jerusalem 9112102, Israel; Institute for Medical Research, Israel-Canada (IMRIC), Ein-Kerem Campus, Jerusalem 9112102, Israel. Electronic address:
Vertebrate oocyte polarity has been observed for two centuries and is essential for embryonic axis formation and germline specification, yet its underlying mechanisms remain unknown. In oocyte polarization, critical RNA-protein (RNP) granules delivered to the oocyte's vegetal pole are stored by the Balbiani body (Bb), a membraneless organelle conserved across species from insects to humans. However, the mechanisms of Bb formation are still unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!