Therminator DNA polymerase, a variant of the 9 degrees N DNA polymerase, is shown to synthesize a functional RNA aptamer; thus providing a simple route for making DNA-tagged RNA aptamers for use in DNA nanotechnology.
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http://dx.doi.org/10.1039/b820678c | DOI Listing |
Circ Res
January 2025
British Heart Foundation Centre for Research Excellence, School of Cardiovascular and Metabolic Medicine and Sciences, James Black Centre, King's College London, United Kingdom (C.Y.H., M.-Y.W., J.T., S.A., L.D., G.A., R.H., C.M.S.).
Background: Vascular calcification is a detrimental aging pathology markedly accelerated in patients with chronic kidney disease. Prelamin A is a biomarker of vascular smooth muscle cell aging that accelerates calcification however the mechanisms remain undefined.
Methods: Vascular smooth muscle cells were transduced with prelamin A using an adenoviral vector and epigenetic modifications were monitored using immunofluorescence and targeted polymerase chain reaction array.
Cureus
December 2024
Department of Clinical Research, University of San Luis Potosí, San Luis Potosí, MEX.
Background: In large-scale molecular studies, a protocol that generates high yields and quality DNA for future polymerase chain reaction (PCR) assays is needed. The collection of buccal cells by cytobrush may represent an efficient, noninvasive, and inexpensive method for obtaining genetic material from school populations. The aim of this study was to develop a method to obtain genomic DNA from buccal cells of schoolchildren, and the DNA was extracted immediately after collecting the buccal cell samples and after storing the samples for 8 months at -20 °C to establish the feasibility of the method for epidemiological studies.
View Article and Find Full Text PDFFront Oncol
January 2025
Department of Biology, Tufts University, Medford, MA, United States.
REV7, also known as MAD2B, MAD2L2, and FANCV, is a HORMA-domain family protein crucial to multiple genome stability pathways. REV7's canonical role is as a member of polymerase ζ, a specialized translesion synthesis polymerase essential for DNA damage tolerance. REV7 also ensures accurate cell cycle progression and prevents premature mitotic progression by sequestering an anaphase-promoting complex/cyclosome activator.
View Article and Find Full Text PDFAm J Clin Exp Immunol
December 2024
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences Gorgan, Iran.
Genetic factors are effective reagents in susceptibility to multiple sclerosis (MS). Previous studies have shown the relationship between heat shock protein (HSP) gene polymorphisms. So, HSP70 single nucleotide polymorphisms (SNPs) were evaluated as MS risk factors.
View Article and Find Full Text PDFBMC Biotechnol
January 2025
CAIQ Center for Biosafety, Chuangyi Rd, Yazhou District, Sanya, Hainan Province, 572024, China.
Background: Food safety is a significant global study subject that is strongly intertwined with human life and well-being. The utilization of DNA-based methods for species identification is a valuable instrument in the field of food inspection and regulation. It is particularly significant for traceability purposes, as it enables the monitoring of a specific item at every level of the food chain regulation.
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