Laboratory animal medicine is a growing field of veterinary practice that emphasizes animal welfare and refinement of research animal care. The Canadian Association for Laboratory Animal Medicine/L'association canadienne de la medecine des animaux de laboratoire (CALAM/ACMAL) and the Canadian Council on Animal Care (CCAC) provide a framework within which laboratory animal veterinarians practise. Numerous continuing education and post-graduate training opportunities exist in Canada for veterinarians interested in pursuing this specialty.
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Invest Ophthalmol Vis Sci
January 2025
Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Purpose: Inflammatory processes have been involved in diabetic retinopathy (DR). Interleukin (IL)-17A, a pro-inflammatory cytokine, is associated with DR occurrence and development. However, mechanisms underlying the IL-17A impact on DR need further investigations.
View Article and Find Full Text PDFInvest Ophthalmol Vis Sci
January 2025
Laboratory of Anatomy of Domestic Animals, National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Haidian, Beijing, China.
Purpose: Because chickens have excellent light perception properties, this study focused on investigating whether monochromatic light can cause photodamage in chicken retinal ganglion cells (RGCs).
Methods: Post-hatching day chickens were exposed to four different light-emitting diode light environments for five weeks, respectively, monochromatic blue light (480 nm), green light (560 nm), red light (660 nm), or white light (6000 K). The mechanisms through which monochromatic light influences the structure of the chicken retina were analyzed by detecting the morphological structure of the retina, gene and protein expression levels, and the ultrastructure of the optic nerve.
Invest Ophthalmol Vis Sci
January 2025
Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Purpose: This study aimed to identify a novel recombinant adeno-associated virus (rAAV) capsid variant that can widely transfect the deep retina through intravitreal injection and to assess their effectiveness and safety in gene delivery.
Methods: By adopting the sequences of various cell-penetrating peptides and inserting them into the capsid modification region of AAV2, we generated several novel variants. The green fluorescent protein (GFP)-carrying variants were screened following intravitreal injection.
EJNMMI Phys
January 2025
Department of Nuclear Medicine, Lanzhou University Second Hospital, Lanzhou, 730030, China.
Purpose: The aim of the study was to investigate the value of SwiftScan Step-and-Shoot Continuous (SSC) scanning mode in enhancing image quality and to explore appropriate scanning parameters for reducing scan time.
Methods: This study was composed of a phantom study and two clinical tests. The differences in visual image quality scores, coefficient of variance (COV) of the background, image signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and recovery coefficient (RC) of the sphere were compared between SSC mode and traditional Step-and-Shoot (SS) mode in the phantom study.
Methods Mol Biol
January 2025
IDG/McGovern Institute of Brain Research, Tsinghua University, Beijing, People's Republic of China.
Mosaic analysis with double markers (MADM) is a powerful in vivo lineage tracing technique. It utilizes Cre recombinase-dependent interchromosomal recombination to restore the stable expression of two fluorescent proteins sparsely in individual dividing stem or progenitor cells and their progenies. Here, we describe the application of this technique for quantitative lineage analysis of radial glial progenitors in the developing mouse neocortex at the single-cell resolution.
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