AI Article Synopsis
- - The proteome of Methylocella silvestris was analyzed using three different proteomic approaches to identify variations in protein expression when grown on two distinct substrates.
- - Results included comparisons of identified proteins based on confidence levels, sequence coverage, and regulated proteins, aiming to assess the reliability of the findings.
- - The study also compared the preparation and execution of the experiments in terms of time, resources, and highlighted initial evaluations of the biological significance of the regulated proteins.
Article Abstract
The proteome of the recently discovered bacterium Methylocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.
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| http://dx.doi.org/10.1021/pr900080y | DOI Listing |
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