The late stages of biosynthesis of phosphinothricin tripeptide (PTT) involve peptide formation and methylation on phosphorus. The exact timing of these transformations is not known. To provide insight into this question, we developed a heterologous expression system for PhsA, one of three NRPS proteins in PTT biosynthesis. The apparent k(cat)/K(m) value for ATP-pyrophosphate exchange activity for d,l-N-acetylphosphinothricin was 3.5 muM(-1) min(-1), whereas the k(cat)/K(m,app) for l-N-acetyldemethylphosphinothricin was 0.5 microM(-1) min(-1), suggesting the former might be the physiological substrate. Each substrate could be loaded onto the phosphopantetheine arm of the thiolation domain as observed by Fourier transform mass spectrometry (FTMS).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709985 | PMC |
| http://dx.doi.org/10.1021/bi900164d | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antibacterial Activity of Peptide Derivatives of Phosphinothricin against Multidrug-Resistant .
Molecules
January 2023
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, 119991 Moscow, Russia.
The fast spread of bacteria that are resistant to many classes of antibiotics (multidrug resistant) is a global threat to human and animal health with a worrisome scenario ahead. Novel therapeutical strategies are of crucial importance to combat this phenomenon. For this purpose, we investigated the antimicrobial properties of the naturally occurring tripeptide Bialaphos and a dipeptide -leucyl--phosphinoithricin, the synthesis and diastereomers separation of which are herein described.
View Article and Find Full Text PDFsp. nov., isolated from bioaerosols of electronic waste.
Int J Syst Evol Microbiol
November 2022
NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Environmental Health, Southern Medical University, Guangzhou 510515, PR China.
Article Synopsis
- A new bacterial strain named GB24 was discovered in bioaerosols at an E-waste recycling site in China, showing it can grow in a range of temperatures (15-40 °C) and pH levels (5.5-9.5).
- It has a unique set of cellular characteristics including specific fatty acids, lipids, and the presence of carotenoid pigments, and is closely related to another strain, DB1506, based on genetic analysis.
- The genomic study of GB24 also shows its potential to produce specific secondary metabolites, leading to its classification as a novel species, proposed to be named sp. nov., with its type strain being GB24.
Genetic Transformation of Common Wheat (Triticum aestivum L.) Using Biolistics.
Methods Mol Biol
February 2021
Plant Sciences Department, Rothamsted Research, Hertfordshire, UK.
The following protocol describes the genetic transformation of wheat using the BioRad PDS/1000-He particle delivery system. Immature embryos are isolated 12-16 days post-anthesis, the embryonic axis is removed, and the immature scutella are precultured for 1-2 days prior to particle bombardment. Gold particles are coated with plasmid DNA containing the gene(s) of interest plus a selectable marker gene, in this instance bar (bialaphos resistance), and are fired into the cells to deliver the DNA.
View Article and Find Full Text PDFConserved biosynthetic pathways for phosalacine, bialaphos and newly discovered phosphonic acid natural products.
J Antibiot (Tokyo)
January 2016
Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Natural products containing phosphonic or phosphinic acid functionalities often display potent biological activities with applications in medicine and agriculture. The herbicide phosphinothricin-tripeptide (PTT) was the first phosphinate natural product discovered, yet despite numerous studies, questions remain surrounding key transformations required for its biosynthesis. In particular, the enzymology required to convert phosphonoformate to carboxyphosphonoenolpyruvate and the mechanisms underlying phosphorus methylation remain poorly understood.
View Article and Find Full Text PDFProteomic approach to reveal the regulatory function of aconitase AcnA in oxidative stress response in the antibiotic producer Streptomyces viridochromogenes Tü494.
PLoS One
October 2014
Department of Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, Eberhard Karls University of Tübingen, Tübingen, Germany.
The aconitase AcnA from the phosphinothricin tripeptide producing strain Streptomyces viridochromogenes Tü494 is a bifunctional protein: under iron-sufficiency conditions AcnA functions as an enzyme of the tricarboxylic acid cycle, whereas under iron depletion it is a regulator of iron metabolism and oxidative stress response. As a member of the family of iron regulatory proteins (IRP), AcnA binds to characteristic iron responsive element (IRE) binding motifs and post-transcriptionally controls the expression of respective target genes. A S.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!