We report here that gemfibrozil (GFZ) inhibits axenic and intracellular growth of Legionella pneumophila and of 27 strains of wild-type and multidrug-resistant Mycobacterium tuberculosis in bacteriological medium and in human and mouse macrophages, respectively. At a concentration of 0.4 mM, GFZ completely inhibited L. pneumophila fatty acid synthesis, while at 0.12 mM it promoted cytoplasmic accumulation of polyhydroxybutyrate. To assess the mechanism(s) of these effects, we cloned an L. pneumophila FabI enoyl reductase homolog that complemented for growth an Escherichia coli strain carrying a temperature-sensitive enoyl reductase and rendered the complemented E. coli strain sensitive to GFZ at the nonpermissive temperature. GFZ noncompetitively inhibited this L. pneumophila FabI homolog, as well as M. tuberculosis InhA and E. coli FabI.
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http://dx.doi.org/10.1128/JB.00175-09 | DOI Listing |
J Water Health
December 2024
Laboklin GmbH & Co KG, Steubenstraße 4, 97688 Bad Kissingen, Germany.
Antimicrobial resistance is an emerging problem in hospitals and long-term healthcare facilities. Early detection of susceptibility pattern changes in pathogenic bacteria can prevent treatment failures. Therefore, this study chose to investigate the antibiotic susceptibility situation of isolates from hospitals and long-term healthcare facilities in Southern Germany.
View Article and Find Full Text PDFJ Water Health
December 2024
Environmental Science, Policy and Research Institute, 144 Narberth Ave, Box 407, Narberth, PA 19072, USA.
is different from traditional drinking water contaminants because it presents a latent public health risk for public and private drinking water systems and for the building water systems they supply. This paper reviews information on the likelihood of occurrence of in public water systems to lay a foundation for public water systems, as a stakeholder in public health risk management, to better manage . Important to this approach is a literature review to identify conditions that could potentially promote being present in drinking water systems at either an elevated abundance or at an increased frequency of occurrence, and/or water quality and supply conditions that would contribute to its amplification.
View Article and Find Full Text PDFJ Water Health
December 2024
US Environmental Protection Agency, Office of Research and Development, Cincinnati, OH 45268, USA.
Hot water systems are the most frequent environment associated with the prevalence and growth of opportunistic premise plumbing pathogens (OPPPs). Previous studies identified water heaters as a source of waterborne diseases and concluded that design variables may contribute to their prevalence. A multifaceted approach was used to investigate the vertical stratification of the microbiome and selected OPPPs in an electric water heater tank connected to a home plumbing system simulator.
View Article and Find Full Text PDFCureus
November 2024
Nephrology, Kanazawa Medical University, Kahoku, JPN.
Legionnaires' disease is a bacterial infection caused by , such as . It mainly causes severe pneumonia, with symptoms such as fever, cough, and shortness of breath. In rare cases, it can cause acute kidney disease and also occasionally become severe enough to require replacement therapy.
View Article and Find Full Text PDFInn Med (Heidelb)
December 2024
Klinik für Innere Medizin, Rheumatologie, Pneumologie, Nephrologie und Diabetologie, medius Klinik Kirchheim, Kirchheim unter Teck, Deutschland.
A 48-year-old male patient was admitted to hospital with Legionella pneumonia. He developed a splenic rupture 1 day after admission, which was surgically treated. Retrospectively, the splenomegaly could already be seen by sonography on admission.
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