AI Article Synopsis

  • A new technology for comparing genomes using NotI-microarrays has been developed, which analyzes tumor and normal DNA to identify genetic changes.
  • The study focused on human chromosome 3, examining 200 tumor samples from various organs and found significant genetic alterations in specific genes linked to cancer.
  • The alterations included deletions and methylation changes, confirmed by additional genetic testing methods, highlighting the potential of this technology for cancer research.

Article Abstract

New comparative genome hybridization technology on NotI-microarrays is presented (Karolinska Institute International Patent WO02/086163). The method is based on comparative genome hybridization of NotI-probes from tumor and normal genomic DNA with the principle of new DNA NotI-microarrays. Using this method 181 NotI linking loci from human chromosome 3 were analyzed in 200 malignant tumor samples from different organs: kidney, lung, breast, ovary, cervical, prostate. Most frequently (more than in 30%) aberrations--deletions, methylation,--were identified in NotI-sites located in MINT24, BHLHB2, RPL15, RARbeta1, ITGA9, RBSP3, VHL, ZIC4 genes, that suggests they probably are involved in cancer development. Methylation of these genomic loci was confirmed by methylation-specific PCR and bisulfite sequencing. The results demonstrate perspective of using this method to solve some oncogenomic problems.

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