AI Article Synopsis
- Cellular aging leads to the build-up of lipofuscin, a type of undegradable material found in lysosomes.
- Researchers cultured human fibroblasts under high oxygen levels to enhance lipofuscin accumulation for their study.
- The findings indicated that lipofuscin-loaded fibroblasts showed more stable lysosomes compared to control cells when stressed, suggesting lipofuscin may help protect lysosomal integrity.
Article Abstract
Cellular ageing is associated with accumulation of undegradable intralysosomal material, called lipofuscin. In order to accelerate the lipofuscin accumulation, confluent, growth-arrested human fibroblasts were cultured under hyperoxic conditions. To provide a better insight into the effects of lipofuscin on cellular functions, we compared lysosomal stability in control and lipofuscin-loaded human fibroblasts under conditions of lysosome-targeted stress induced by exposure to either the lysosomotropic detergent MSDH or the redox-cycling quinone naphthazarin. We show that lysosomal damage, assessed by acridine-orange relocation, translocation of cathepsin D to the cytosol, and alkalinization of lysosomes, is more pronounced in control than in lipofuscin-loaded fibroblasts. Finding that lysosomal integrity was less affected or even preserved in case of lipofuscin-loaded cells enables us to suggest that lipofuscin exerts lysosome-stabilizing properties.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276591 | PMC |
| http://dx.doi.org/10.1007/s11357-007-9045-9 | DOI Listing |
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