This paper presents the effect of ethylene diamine tetraacetic acid (EDTA) as a washing solution on the electrokinetic process for removal of Cr, Pb and Zn from sewage sludge. The sequential chemical extraction scheme according to the guidelines of BCR (Community Bureau of Reference) was applied to the sludge samples to evaluate the effect of EDTA on metal fractionation during electrokinetic processes. The highest removals of the heavy metals were 34% for Cr, 27% for Pb and 20% for Zn with 0.1N EDTA. The removal priority of the metals by electrokinetic process was found to be Cr>Pb>Zn. According to the results of BCR analysis, addition of EDTA did not create the inter-transformation of Cr, Pb and Zn although the metal concentration decreased.
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http://dx.doi.org/10.1016/j.jhazmat.2009.04.008 | DOI Listing |
Biol Methods Protoc
October 2024
One Health Research Group, Facultad de Ciencias de la Salud, Universidad de Las Américas, Quito, 170124, Ecuador.
We present a rapid high-throughput DNA extraction method for use with EDTA-anticoagulated blood using silicon dioxide (SiO) powder in a guanidine-HCl solution, hereinafter referred to as "Glassmilk." The method utilizes a 96-well deep-well plate, enabling DNA extraction from 96 samples in under 3 h. The method integrates cell lysis, washing, elution, and DNA storage within the same well, eliminating the need for DNA transfer.
View Article and Find Full Text PDFJ Hazard Mater
December 2024
Department of Sustainable Land Management & Soil Research Centre, School of Agriculture, Policy and Development, University of Reading, Reading, Berkshire, UK.
Monitoring heavy metals in vegetation near mining or industrial sites is crucial for detecting plant contamination; requiring discrimination between metals adhered to foliar surfaces from the internal concentrations. We investigated key factors that might contribute to lead (Pb) accumulation in leaves of local vegetation near a Pb mine: (i) distance from the pollutant source, (ii) morphological characteristics of leaf surfaces, (iii) their susceptibility to Pb loss by washing, and (iv) the effect of contrasting washing reagents in Pb removal. Native plant species were sampled at three field locations, possessing different leaf surface morphologies: glabrous (smooth), resinous (waxy) and hirsute (hairy).
View Article and Find Full Text PDFJ Hazard Mater
December 2024
Key Laboratory of Rare Earths, Jiangxi Province Key Laboratory of Cleaner Production of Rare Earths, Ganjiang Innovation Academy, Chinese Academy of Sciences, Ganzhou 341119, China; CAS Key Laboratory of Green Process and Engineering, National Engineering Research Center of Green Recycling for Strategic Metal Resources, Innovation Academy for Green Manufacture, Beijing Engineering Research Centre of Process Pollution Control, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; School of Metallurgical engineering, Jiangxi University of Science and Technology, Ganzhou 341099, China; School of Rare Earths, University of Science and Technology of China, Hefei 230026, China; State Key Laboratory of Biochemical Engineering, Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:
Coal gangue, a solid waste generated during coal mining and washing processes, has caused significant environmental burdens in China. This study aims to optimize and investigate the leaching mechanisms of heavy metals, such as Pb, Zn, and Cu, in coal gangue. The effectiveness of different eluents in removing heavy metals from coal gangue was evaluated by combining experimental methods with software simulations.
View Article and Find Full Text PDFToxins (Basel)
November 2024
Laboratório de Matriz Extracelular e Biotecnologia de Venenos, Universidade Federal do Paraná, UFPR, Curitiba 81531-980, Brazil.
J Vis Exp
November 2024
Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile;
During placenta development, extravillous trophoblast (EVT) cells invade the maternal decidua to remodel the uterine spiral arteries by a process of mesenchymal to endothelial-like transition. Traditionally, this process is evaluated by an in vitro tube-formation assay, where the cells organize themselves into tube-like structures when seeded over a polymerized basement membrane preparation. Although several structural features can be measured in photomicrographs of the structures, to assess the real commitment of EVT to the endothelial-type phenotype, biochemical analysis of cell extracts is required.
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