AI Article Synopsis
- * In tests with K562 cells and a pWizGFP plasmid, SFE achieved better transgene expression (10-15%) than a commercial system while maintaining over 50% cell viability.
- * The improvements were due to reduced gas formation and Joule heating during the SFE process, and similar results were observed with mouse embryonic stem cells.
Article Abstract
We have recently developed a semicontinuous flow electroporation (SFE) device for in vitro DNA delivery. Cells mixed with plasmid DNA continuously flowed through a serpentine channel, the side walls of which also serving as electrodes. With the use of pWizGFP plasmid and K562 cells as a model system, SFE showed better transgene expression (10-15%) compared to a commercial electroporation system. Quantitative results via MTS assay also revealed a 50% or higher cell viability. Similar observations were also found with pWizGFP transfection to mouse embryonic stem cells. Such improvements were attributed to less gas formation and Joule heating in SFE.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700304 | PMC |
| http://dx.doi.org/10.1021/ac9002672 | DOI Listing |
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