Background: The increasing occurrence of Qo inhibitor (QoI)-fungicide-resistant Plasmopara viticola (Berk. & MA Curtis) Berl. & DeToni populations is becoming a serious problem in the control of grapevine downy mildew worldwide.
Results: The authors have developed a rapid method for detecting resistance to a QoI fungicide, azoxystrobin, in P. viticola populations using the nested PCR-RFLP method. With this method, a glycine-to-alanine substitution was discovered at codon 143 in the cytochrome b gene of P. viticola populations found in Japan.
Conclusion: It is proposed that the nested PCR-RFLP method is a high-speed, sensitive and reliable tool for detecting azoxystrobin-resistant P. viticola populations.
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| http://dx.doi.org/10.1002/ps.1765 | DOI Listing |
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