Objective: To detect changes in serum lipoprotein and apolipoprotein profiles via precipitation and electrophoresis in ketotic cows and in those cows treated with different methods.
Animals: 21 cows with clinical and subclinical ketosis, 7 healthy cows in the early lactation period, and 7 healthy cows in the nonlactation period.
Procedures: Ketotic cows were allocated into 3 groups; the first group was treated with dextrose and dexamethasone, the second group with dextrose and prednisolone, and the third group with dextrose and insulin. The beta and alpha lipoproteins were precipitated with dextran sulfate-magnesium chloride in ketotic cows after treatment and healthy cows in the nonlactation and lactation periods. The serum samples, precipitates, and supernatants were examined via agarose gel electrophoresis for detection of alterations in serum lipoproteins. Subsequently, alterations in serum apolipoproteins were detected via SDS-PAGE of precipitates.
Results: Compared with serum beta and alpha lipoprotein concentrations in healthy cows during nonlactation, those in cows during lactation were higher; however, those in cows with ketosis were lower. The SDS-PAGE analysis of serum beta lipoproteins revealed that apolipoprotein E (approx 36 and 40 kDa) decreased in ketotic cows, in comparison with healthy cows in the nonlactation and lactation periods, but increased after treatment. Decreases in apolipo-protein B (approx 222 kDa), apolipoprotein A-I (19 and 24 kDa), apolipoprotein A-IV (55 kDa), apolipoprotein C-III (8.8 and 10.2 kDa), and albumin (66 kDa) concentrations were detected in ketotic cows, in comparison with the healthy cows in the lactation period.
Conclusions And Clinical Relevance: Serum lipoprotein and apolipoproteins may routinely be determined via precipitation and electrophoresis in the diagnosis and treatment of ketosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2460/ajvr.70.5.563 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Safety of Multiple-Dose Liquid Blend Containing Kava and Kratom in Healthy Adults.
Cureus
December 2024
Clinical Pharmacology, Botanic Tonics, LLC, Calabasas, USA.
This study investigates the safety of three different daily dosages of a liquid blend containing kava and kratom (Feel Free® Classic Tonic {FFCT}) in healthy adults over six consecutive days of supplementation. Both kava and kratom have been used traditionally for hundreds of years, but there is limited data on the combined safety of these ingredients. In this randomized, double-blind, placebo-controlled trial, the participants were assigned to receive one of three daily dosages of FFCT or placebo.
View Article and Find Full Text PDFRelative increase in walking activity in lactating dairy cows submitted to timed artificial insemination: Risk factors and subsequent fertility.
Anim Reprod Sci
January 2025
Agrotecnio Center, Department of Animal Sciences, University of Lleida, Lleida 25198, Spain.
The objectives of this study were: (i) to evaluate the associations between postpartum health disorders, relative increase in walking activity (RIWA) and expression of behavioral estrus intensity captured by pedometers; (ii) to assess associations between RIWA and pregnancy in cows submitted to timed artificial insemination (TAI). Lactating dairy cows (n = 881) were enrolled in the study. Cows were submitted to a 5d TAI protocol with insertion of a progesterone device.
View Article and Find Full Text PDFNeurofilament light chain (Nf-L) in cerebrospinal fluid and serum as a potential biomarker in the differential diagnosis of neurological diseases in cattle.
Vet Res
January 2025
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2-5, 10095, Grugliasco, TO, Italy.
Article Synopsis
- Neurofilament light chain (Nf-L) is identified as a potential biomarker for diagnosing neurological disorders in cattle, paralleling its established role in human neurology.
- The study found that Nf-L levels varied significantly between healthy and sick cattle, with consistent median levels detected in serum and cerebrospinal fluid (CSF) across different age groups.
- There is a promising association between Nf-L levels in serum and CSF, particularly in cattle with neurological disorders, indicating that Nf-L could serve as a valuable diagnostic tool in veterinary practices.
Persistently high concentrations of β-hydroxybutyrate affect hepatic SOD2 expression and blood SOD activity in high-yielding dairy cows.
BMC Vet Res
January 2025
Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, 02-787, Poland.
Background: Elevated BHB levels are hypothesized to influence hepatic antioxidant enzyme expression and activity, contributing to oxidative response. However, the impact of BHB between 0.8 and 1.
View Article and Find Full Text PDFInternational Symposium on Ruminant Physiology: The immunometabolism of transition dairy cows from dry-off to early lactation: lights and shadows.
J Dairy Sci
January 2025
Department of Animal Science, Food and Nutrition (DIANA), Faculty of Agricultural, Food and Environmental Sciences, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy.
The mismatch between the nutrient intake from the diet and the output by the mammary gland causes a negative energy balance in transition dairy cows, that, if excessive, can promote several metabolic disorders. Other relevant phenomena occur during transition, such as inflammation at calving and changes in immunocompetence, redox balance, and mineral metabolism. Despite the efforts, some aspects of the adaptive mechanisms observed in the transition period still need to be clarified.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!