AI Article Synopsis
- Chicory roots are rich in inulin, a carbohydrate beneficial for both plants and humans, serving as a dietary and prebiotic component.
- A 2-D electrophoresis study identified 881 protein spots, with 714 matching database entries; 619 of these proteins were classified based on their functions.
- Key functional categories included metabolism, stress response, and stability, highlighting the significant role of cold acclimation proteins due to the low temperatures experienced before harvest.
Article Abstract
Chicory (Cichorium intybus) roots contain high amounts of inulin, a fructose polymer used as a storage carbohydrate by the plant and as a human dietary and prebiotic compound. We performed 2-D electrophoretic analysis of proteins from root material before the first freezing period. The proteins were digested with trypsin and the peptides analyzed by MS (MALDI-TOF/TOF). From the 881 protein spots analyzed, 714 proteins corresponded to a database accession, 619 of which were classified into functional categories. Besides expected proteins (e.g. related to metabolism, energy, protein synthesis, or cell structure), other well-represented categories were proteins related to folding and stability (49 spots), proteolysis (49 spots), and the stress response (67 spots). The importance of abiotic stress response was confirmed by the observation that 7 of the 21 most intense protein spots are known to be involved in cold acclimation. These results suggest a major effect of the low temperature period that preceded root harvesting.
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| http://dx.doi.org/10.1002/pmic.200800744 | DOI Listing |
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