Discovery and analysis of a novel parvovirus B19 Genotype 3 isolate in the United States.

Background: Parvovirus B19 (B19V) is a pathogen frequently identified in human plasma donations through the detection of nucleic acids. Three B19V genotypes have been defined based on isolates having greater than 10% divergence in overall DNA sequence. B19V Genotype 3 is a rarely occurring genotype that has been detected primarily in Ghana with sporadic reports in Brazil and France but has not been previously reported in North America.

Study Design And Methods: A polymerase chain reaction assay was developed with broad specificity for B19V detection. The performance of this assay was assessed by testing approximately 440,000 clinical samples representing more than 81,000 individual donors. Determinations of B19V titer, DNA sequence, and antibody concentrations were performed on samples of interest.

Results: This assessment identified a series of eight plasma donations spanning 28 days from a single donor in the United States infected with B19V Genotype 3 as confirmed by DNA sequence analysis. The B19V titer of this series of donations showed virus titers that peaked at greater than 10(11) IU/mL. The virus titer decreased significantly over the next several donations coinciding with an increase in immunoglobulin M (IgM) levels. The immunoglobulin G levels also increased but lagged approximately 7 days behind the IgM levels.

Conclusion: This is the first report of a B19V Genotype 3 detected from a plasma donor located in the United States. Although our data are consistent with recent reports suggesting low incidence for this genotype, they indicate its increasing relevance among blood and plasma donors.