The interactions of cobalt(II)-4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene (5-Cl-PADAB) complex with different kinds of homopolymer oligonucleotides in basic medium were investigated based on the measurements of resonance light scattering, UV-vis, circular dichroism spectra and dark field light-scattering imaging. Experiments showed that only thymidine homopolymer (poly T) oligonucleotides with the length in the range of poly T6 to poly T18 could interact with the Co(II)-5-Cl-PADAB complex in alkaline conditions and cause evident color and spectral change. Thus, the binary complex of Co(II)-5-Cl-PADAB could be employed as a visual probe for selectively recognizing the poly T oligonucleotides.
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