We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691077 | PMC |
| http://dx.doi.org/10.1128/JCM.00702-09 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of VP4-VP2 sequencing for molecular typing of human enteroviruses.
PLoS One
December 2024
Department of Virology II, National Institute of Infectious Diseases, Musashi-murayama, Tokyo, Japan.
Enteroviruses and rhinoviruses are highly diverse, with over 300 identified types. Reverse transcription-polymerase chain reaction (RT-PCR) assays targeting their VP1, VP4, and partial VP2 (VP4-pVP2) genomic regions are used for detection and identification. The VP4-pVP2 region is particularly sensitive to RT-PCR detection, making it efficient for clinical specimen analysis.
View Article and Find Full Text PDFNon-lytic spread of poliovirus requires the nonstructural protein 3CD.
mBio
January 2025
Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Unlabelled: Non-enveloped viruses like poliovirus (PV) have evolved the capacity to spread by non-lytic mechanisms. For PV, this mechanism exploits the host secretory autophagy pathway. Virions are selectively incorporated into autophagosomes, double-membrane vesicles that travel to the plasma membrane, fuse, and release single-membrane vesicles containing virions.
View Article and Find Full Text PDFAssessing the basis for regulatory crediting of virus LRVs for secondary biological wastewater treatment: A systematic review.
Water Res
November 2024
Southern Nevada Water Authority, P.O. Box 99954, Las Vegas, NV 89193, United States. Electronic address:
Regulatory frameworks for potable reuse often include stringent log reduction value (LRV) targets to ensure public health protection against exposure to viruses and protozoa. To achieve overall LRV targets and reduce associated capital and operational costs, it is important to maximize LRV credits awarded to each unit process in a potable reuse treatment train. This may include processes that are historically uncredited or undercredited, such as secondary biological wastewater treatment incorporating activated sludge and secondary clarification.
View Article and Find Full Text PDFHarnessing the Power of Next-Generation Sequencing in Wastewater-Based Epidemiology and Global Disease Surveillance.
Food Environ Virol
November 2024
School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.
Article Synopsis
- Wastewater-based epidemiology (WBE) is a valuable tool for monitoring community infections, including silent cases of SARS-CoV-2 and other pathogens.
- Next-generation sequencing (NGS) allows for more detailed analyses of viruses, but many facilities still don't use these advanced methods in their routine surveillance.
- The review discusses how to better incorporate NGS into WBE programs to improve detection of emerging viruses and enhance global health response strategies.
Antiviral Development for the Polio Endgame: Current Progress and Future Directions.
Pathogens
November 2024
Poliovirus & Picornavirus Branch, Division of Viral Diseases, National Center for Immunization and Other Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
As the world is approaching the eradication of wild poliovirus serotype 1, the last of the three wild types, the question of how to maintain a polio-free world becomes imminent. To mitigate the risk of sporadic vaccine-associated paralytic polio (VAPP) caused by oral polio vaccines (OPVs) that are routinely used in global immunization programs, the Polio Antivirals Initiative (PAI) was established in 2006. The primary goal of the PAI is to facilitate the discovery and development of antiviral drugs to stop the excretion of immunodeficiency-associated vaccine-derived poliovirus (iVDPV) in B cell-deficient individuals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!