Determination of the proportion of neutrophils in the peripheral blood is important for diagnostic purposes in medicine and for evaluating new drugs in the pharmaceutical industry. To measure the neutrophil concentration in rat blood, a fast and accurate flow cytometric method was developed. Rat neutrophils were quantified by using primary antibodies that recognize the RP1 antigen and secondary antibodies conjugated with fluorescein isothiocyanate. The flow cytometric method was calibrated by comparing cytometric results with data from a manual differential count. The results obtained by these 2 methods correlated with a Pearson correlation coefficient of 0.91 and were in agreement according to subsequent statistical analysis. To confirm the usefulness of the method in preclinical applications, the production of neutrophils in rats was stimulated by pegfilgrastim. Blood samples were taken at predetermined time points, and the pharmacodynamic profile was determined. These results confirmed that the flow cytometric method for neutrophil quantification is accurate and much faster than the manual microscopic method. Moreover, the flow cytometric method is easy to use, suggesting that it could become the method of choice for preclinical applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2679661 | PMC |
Publication Analysis
Top Keywords
Similar Publications
The therapeutic role of naringenin nanoparticles on hepatocellular carcinoma.
BMC Pharmacol Toxicol
January 2025
Biochemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.
Background: Naringenin, a flavonoid compound found in citrus fruits, possesses valuable anticancer properties. However, its potential application in cancer treatment is limited by poor bioavailability and pharmacokinetics at tumor sites. To address this, Naringenin nanoparticles (NARNPs) were prepared using the emulsion diffusion technique and their anticancer effects were investigated in HepG2 cells.
View Article and Find Full Text PDFSerum starvation as the most suitable method for inducing G/G phase cell cycle arrest in six-banded armadillo (Euphractus sexcinctus) skin-derived fibroblast lines.
Tissue Cell
December 2024
Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, Brazil. Electronic address:
Background: Several studies have evaluated different cell cycle synchronization methods to improve reprogramming efficiency aimed at wild species conservation. The six-banded armadillo is one of the wild mammals with significant ecological and biomedical interests but has not yet been evaluated for reprogramming purposes.
Objective: We investigated the effects in a time-dependent manner of serum starvation (SS; 0.
Astragaloside IV confers neuroprotection against radiation-induced neuronal senescence via the ERK pathway.
Exp Neurol
December 2024
School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu Province, China. Electronic address:
Various factors and mechanisms, including radiation, initiate cellular senescence and are concurrent with the progression of various neurodegenerative diseases. Radiation-induced chromosomal aberrations and DNA integrity damage impact the processes of cellular growth, maturation, and aging. Astragaloside IV (AS-IV) has been documented to display significant neuroprotective effects on inflammation, oxidative stress, and cellular apoptosis; however, the precise neuroprotective mechanism of AS-IV against neuronal aging remains unclear.
View Article and Find Full Text PDFLactamase β reprograms lipid metabolism to inhibit the progression of endometrial cancer through attenuating MDM2-mediated p53 ubiquitination and degradation.
Arch Biochem Biophys
December 2024
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Laboratory of Laparoscopic Technology, the First Affiliated Hospital of Shandong First Medical University, Jinan, 250014, Shandong, China. Electronic address:
Background: Lactamase β (LACTB) inhibits the metastasis and progression of multiple malignant tumors. However, little is known about its role in endometrial cancer (EC). Our study aimed to investigate the function and potential molecular mechanism of LACTB in modulating EC progression.
View Article and Find Full Text PDFA novel technique to characterize procoagulant platelet formation and evaluate platelet procoagulant tendency in cats by flow cytometry.
Front Vet Sci
December 2024
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.
Introduction: Cardiogenic arterial thromboembolism (CATE) is a life-threatening complication of hypertrophic cardiomyopathy (HCM) with a high mortality rate. As the primary responders in hemostasis, platelets play a crucial role in the progression of CATE. Procoagulant platelets are a subpopulation of activated platelets that facilitate thrombin generation to strengthen thrombus structure.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!