AI Article Synopsis
- Overexpression of N-cadherin in mouse embryonic stem cells resulted in significant changes in cell characteristics, including smaller embryonic bodies and increased levels of N-cadherin mRNA and protein.
- The study found that these N-cadherin overexpressing cells had reduced attachment to non-cell surfaces but enhanced adhesiveness to one another and to rat neonatal heart cells.
- These results indicate that boosting N-cadherin could improve the integration of stem cells into tissues like the heart and brain, which naturally have high levels of N-cadherin.
Article Abstract
Constitutive overexpression of N-cadherin in mouse embryonic stem cells led to marked changes in the phenotype and adhesion properties of these cells. The changes included the formation of smaller embryonic bodies, elevated mRNA and total protein levels of N-cadherin, and increased amounts of p120 catenin and connexin-43. N-cadherin cells exhibited decreased attachment to non-cell surfaces, while their adhesiveness to each other and to rat neonatal cardiomyocytes was significantly elevated. The findings suggest that N-cadherin overexpression can facilitate electromechanical integration of stem cells into excitable tissues with endogenously high levels of N-cadherin, such as the heart and brain.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712817 | PMC |
| http://dx.doi.org/10.4161/cam.3.3.8399 | DOI Listing |
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