A unique group I intron in Coxiella burnetii is a natural splice mutant.

J Bacteriol

Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA.

Published: June 2009

AI Article Synopsis

  • Cbu.L1917 is a unique group I intron found in the 23S rRNA gene of the bacteria Coxiella burnetii, characterized by having an adenine instead of a typical guanine at its 3' end.
  • Unlike other group I introns, Cbu.L1917 uses distinct cofactors for each step of its splicing process.
  • It also exhibits a slower self-splicing rate when tested in vitro compared to other similar introns.

Article Abstract

Cbu.L1917, a group I intron present in the 23S rRNA gene of Coxiella burnetii, possesses a unique 3'-terminal adenine in place of a conserved guanine. Here, we show that, unlike all other group I introns, Cbu.L1917 utilizes a different cofactor for each splicing step and has a decreased self-splicing rate in vitro.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698393PMC
http://dx.doi.org/10.1128/JB.00359-09DOI Listing

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