AI Article Synopsis
- The study examined how cobratoxin (CTX) impacts pain-related neuron activity in the thalamic parafascicular nucleus of rats, finding a dose-dependent decrease in pain-evoked discharges with higher doses of CTX.
- CTX maintained its inhibitory effects on neuron activity for at least 2 hours at the highest dose, whereas morphine only had a lasting effect of about 30 minutes.
- The research indicated that CTX's pain relief mechanisms involve central cholinergic and serotonergic pathways, but not the opioidergic system, as demonstrated by the reversal of its effects with atropine and impact from tryptophan hydroxylase inhibition.
Article Abstract
The present study investigated the inhibitory effect of cobratoxin (CTX) on pain-evoked discharge of neurons in thalamic parafascicular nucleus (Pf) of rats and analyzed some of the mechanisms involved in this effect. Intracerebroventricular injection (icv) of CTX at 0.56, 1.12 and 4.50 microg/kg resulted in a dose-dependent inhibitory effect on the pain-evoked discharges of Pf neurons. The inhibition of pain-evoked discharges of Pf neurons by CTX at high dose (4.50 microg/kg) persisted at least for 2h, while the inhibitory effect of morphine (40 microg) persisted no longer than 30 min. The inhibitory effect of CTX was reversed by pretreatment with atropine (icv, 5 microg). In contrast, icv injection of naloxone (4 microg) had no effect on CTX-induced inhibition. Furthermore, pretreatment with parachlorophenylalanine, a specific inhibitor of tryptophan hydroxylase, also significantly attenuated the inhibitory effect of CTX. The results suggested that: (a) CTX has a dose-dependent inhibitory effect on pain-evoked discharges of Pf neurons, confirming electrophysiologically the antinociceptive action of CTX; (b) the inhibitory effect of CTX has a longer duration compared to that of morphine; (c) central cholinergic and serotonergic systems, but not opioidergic system, are involved in the inhibitory effect of CTX.
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| http://dx.doi.org/10.1016/j.toxicon.2009.04.007 | DOI Listing |
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