Background: Clinical manifestations suggest that air pollution may induce deterioration of respiratory health. Some air pollutants, including sulfite, may play a role in the exacerbation of asthma. Sulfites are formed at bronchial mucosa from inhaled sulfur dioxide. It has been previously reported that sodium sulfite (Na(2)SO(3)) has pro-inflammatory properties and enhances neutrophil adhesion to A549 cells. Interleukin-8 (IL-8) plays a critical role in attracting inflammatory cells and is an excellent marker of pulmonary cell activation. To date, there have not been any reports on the effect of asthma drugs on the suppression of IL-8 production induced by sulfite in A549 cells or the involvement of specific signal transduction pathways. Thus, our study assessed the effects of salmeterol, fluticasone, and montelukast on human epithelial lung cell inflammation as well as the inhibitors in different signal transduction pathways.
Methods: A549 human lung epithelial cells were cultured under the following conditions: (1) treated with sodium sulfite (0, 100, 500, 1000, 2500 uM) for 16 hours; (2) cultured for 1 hour in the presence of SB203580, PD98059, SP600125, or wedeloactone, then co-incubated with sodium sulfite for another 16 hours; (3) cultured for 4 hours in the presence of salmeterol, fluticasone, or montelukast, then stimulated with sodium sulfite at a concentration of 1000 uM for 16 hours. We collected the supernatants from the above conditions and performed enzyme-linked immunosorbent assay (ELISA) to measure the IL-8 concentration.
Results: IL-8 production increased after treatment with sodium sulfite at 1000 to 2500 uM (p
Conclusions: Sodium sulfite has pro-inflammatory properties in vitro and can induce potent chemotactic factor IL-8 production. Possible signal transduction pathways required for IL-8 gene expression following exposure to sulfite are the NF-kappa B, ERK, and p-38-dependent pathways. Salmeterol, fluticasone, and montelukast all have inhibitory effects on sodium sulfite-induced IL-8 production in A549 cells.
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http://dx.doi.org/10.1080/02770900802628508 | DOI Listing |
Membranes (Basel)
January 2025
Godo Shigen Co., Ltd., 1545-1 Nanaido, Chosei-mura, Chiba 299-4333, Japan.
Iodine, being an important resource, must be recovered and reused. Iodine is not only attracted to the hydrophobic silicone membrane but also easily vaporized. In this study, we explored the use of five types of silicone hollow fiber membrane modules (SFMMs) for separating iodine in the gaseous phase.
View Article and Find Full Text PDFPLoS One
January 2025
Group of Research in Ruminants (G2R), Department of Animal and Food Sciences, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Fiber is essential for rumen health, microbial fermentation, and the energy supply of herbivores. Even though the study of fecal fiber contents (neutral detergent fiber NDF, acid detergent fiber ADF, and acid detergent lignin ADL) using near-infrared reflectance spectroscopy (NIRS) has allowed investigating nutritional ecology of different herbivore species, NIRS calibrations are species-specific and require a large number of samples for predictions. A multispecies calibration would be an advantage since samples from different herbivores could be used to calibrate a model capable of predicting the fecal fiber content of other herbivores.
View Article and Find Full Text PDFWater Res
March 2025
Hydrochemistry Research Laboratory, Department of Chemical Sciences, Adekunle Ajasin University, Akungba, Nigeria.
This study investigates the catalytic potential of anionic MnO nanosheets (Mn), a cationic covalent organic Framework (COF), and their composites (CM, C2M, and CM2) with varying Mn weight percentages for arsenic removal from groundwater. The catalysts were used as activators of sodium sulfite (SS) under light conditions, enabling the pre-oxidation of arsenic (III) (As3) to arsenic(V) (As5), followed by the adsorption of As5 for complete arsenic removal from water. The Mn-COF-SS systems (C2M, CM, CM2) exhibited superior performance compared to Mn and COF alone, highlighting the dual functionality of the composites as both oxidizing agents and effective adsorbents for arsenic species.
View Article and Find Full Text PDFAppl Biochem Biotechnol
December 2024
Department of Bioinformatics, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai, 602105, Tamil Nadu, India.
Food preservation aims to maintain safe and nutritious food for extended periods by inhibiting microbial growth that causes spoilage and poses health risks. Traditional chemical preservatives like sodium sulfite, sodium nitrite, sodium benzoate, tBHQ and BHA have raised concerns due to potential carcinogenicity, genotoxicity and allergies with long-term consumption. As a natural alternative, bacteriocins have emerged for food preservation.
View Article and Find Full Text PDFAnal Chem
November 2024
State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Earth Sciences, Chengdu University of Technology, Chengdu, Sichuan 610059, China.
A highly sensitive method for the determination of total arsenic (As) has been developed using photochemical vapor generation (PVG) coupled with inductively coupled plasma mass spectrometry (ICP MS). The efficient PVG of As is reported for the first time in the presence of Cd(II) in diluted formic acid (FA) medium. The PVG efficiency of 93 ± 1% can be achieved in the system with 0.
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