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Human polyomavirus DNA detection in keratoacanthoma and Spitz naevus: no evidence for a causal role.
J Clin Pathol
May 2017
Department of oncology and pathology, Karolinska Institutet, Stockholm, Sweden.
Keratoacanthomas (KA) and Spitz naevus (SN) are both lesions with unknown aetiology; therefore, the possibility of a viral involvement, more specifically the involvement of human polyomaviruses (HPyV), was investigated. In total, 22 cases of KA and 25 cases of SN were tested for the presence of HPyVs. DNA was extracted and amplified by multiplex PCR and thereafter tested with a multiplex bead-based assay for HPyVs (BKPyV, JCPyV, KIPyV, WUPyV, MCPyV, TSPyV, HPyV6, 7 and 9) and two primate viruses (SV40 and LPyV).
View Article and Find Full Text PDFImmunohistochemical staining of cutaneous tumours with G-81, a monoclonal antibody to dermcidin.
Br J Dermatol
July 2004
Department of Dermatology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, Japan.
Background: Recently, the novel antimicrobial peptide named dermcidin (DCD) was reported in human eccrine sweat glands.
Objectives: We investigated the expression of DCD in a variety of cutaneous tumours in order to assess the usefulness of the monoclonal antibody (G-81), which recognizes a fragment of DCD.
Patients/methods: We studied the immunoreactivity of the G-81 antibody on 197 cutaneous tumours.
[AgNOR expression in skin tumors. Studies of melanocytic, epidermal and fibrohistiocytic lesions].
Hautarzt
March 1995
Klinik und Poliklinik für Hautkrankheiten, Medizinischen Fakultät Carl Gustav Carus, Technischen Universität, Dresden.
Nucleolus organizer regions are segments of DNA-coding ribosomal genes, which can be visualized histologically by a special silver staining technique as so-called AgNORs. Determination of the number and size of AgNORs provides quite a precise reflection of current cellular proliferation activity. We have examined the AgNOR expression in 228 melanocytic, epidermal and fibrohistiocytic lesions.
View Article and Find Full Text PDF[Use of the AgNOR method for the differential diagnosis of tumors and for the quantification of non-neoplastic epidermal hyperproliferation in dermatohistology].
Zentralbl Pathol
March 1994
Praxis für Pathologie, Radeberg, Carl Gustav Carus, Dresden, Deutschland.
The AgNOR number (NN) and the AgNOR quotient (NQ) of 228 melanocytic, epidermal and fibrohistiocytic lesions were investigated by image analysis. In melanocytic nevi, dysplastic nevi and Spitz nevi, significantly less AgNORs were seen than in malignant melanomas and melanomas in situ. Significant differences in AgNOR expression were detected between the subtypes of melanoma, too.
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