Cellular development is highly influenced by the surrounding microenvironment. We propose user-reconfigurable microenvironments and bio-compatible scaffolds as an approach for understanding cellular development processes. We demonstrate a model platform for constructing versatile microenvironments by fabricating morphologically complex microstructures by two-photon polymerization (2PP) and then assembling these archetypal building blocks into various configurations using multiple, real-time configurable counterpropagating-beam (CB) traps. The demonstrated capacity for handling feature-rich microcomponents may be further developed into a generalized microassembly platform.
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http://dx.doi.org/10.1364/oe.17.006578 | DOI Listing |
Micromachines (Basel)
February 2024
Mechanical and Aerospace Engineering, University of California Irvine, 5200 Engineering Hall, Irvine, CA 92627, USA.
The presented study demonstrates the capability of the template-based electrokinetic assembly (TEA) and guidance to manipulate and capture individual biological cells within a microfluidic platform. Specifically, dielectrophoretic (DEP) focusing of K-562 cells towards lithographically-defined "wells" on the microelectrodes and positioning singles cells withing these "wells" was demonstrated. K-562 lymphoblast cells, are widely used in immunology research.
View Article and Find Full Text PDFSci Robot
August 2022
FEMTO-ST Institute, CNRS, Univ. Bourgogne Franche-Comté, 24 rue Alain Savary, F-25000 Besançon, France.
Although robotic micromanipulation using microtweezers has been widely explored, the current manipulation throughput hardly exceeds one operation per second. Increasing the manipulation throughput is thus a key factor for the emergence of robotized microassembly industries. This article presents MiGriBot (Millimeter Gripper Robot), a miniaturized parallel robot with a configurable platform and soft joints, designed to perform pick-and-place operations at the microscale.
View Article and Find Full Text PDFMicrosyst Nanoeng
June 2021
Wyant College of Optical Sciences, The University of Arizona, Tucson, Arizona 85721 USA.
The fabrication of three-dimensional (3D) microscale structures is critical for many applications, including strong and lightweight material development, medical device fabrication, microrobotics, and photonic applications. While 3D microfabrication has seen progress over the past decades, complex multicomponent integration with small or hierarchical feature sizes is still a challenge. In this study, an optical positioning and linking (OPAL) platform based on optical tweezers is used to precisely fabricate 3D microstructures from two types of micron-scale building blocks linked by biochemical interactions.
View Article and Find Full Text PDFSci Robot
March 2021
Transfers Interfaces and Processes (TIPs), Ecole Polytechnique de Bruxelles (CP 165/67), Université Libre de Bruxelles, 1050 Brussels, Belgium.
Future developments in micromanufacturing will require advances in micromanipulation tools. Several robotic micromanipulation methods have been developed to position micro-objects mostly in air and in liquids. The air-water interface is a third medium where objects can be manipulated, offering a good compromise between the two previously mentioned ones.
View Article and Find Full Text PDFMicromachines (Basel)
December 2019
Department of Mechanical and Industrial Engineering, Northeastern University, Boston, MA 02115, USA.
Modular microfluidics offer the opportunity to combine the precise fluid control, rapid sample processing, low sample and reagent volumes, and relatively lower cost of conventional microfluidics with the flexible reconfigurability needed to accommodate the requirements of target applications such as drug toxicity studies. However, combining the capabilities of fully adaptable modular microelectromechanical systems (MEMS) assembly with the simplicity of conventional microfluidic fabrication remains a challenge. A hybrid polydimethylsiloxane (PDMS)-molding/photolithographic process is demonstrated to rapidly fabricate LEGO-like modular blocks.
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