AI Article Synopsis
- A new type of microscope has been created that uses surface plasmons to enhance two-photon total-internal-reflection fluorescence (TIRF) for better imaging of living cell membranes.
- * This microscope provides brighter images and reduces photobleaching thanks to its ability to enhance the local electromagnetic field and having shorter fluorophore lifetimes.
- * It demonstrates higher signal-to-noise ratios and improved image quality when imaging living monkey kidney cells compared to traditional one-photon TIRF techniques.
Article Abstract
A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.
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| http://dx.doi.org/10.1364/oe.17.005987 | DOI Listing |
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