AI Article Synopsis
- Anidulafungin is an antifungal drug that targets the cell walls of Candida species by blocking beta-1,3-glucan synthase, leading to cell death and lasting effects even after the drug is removed.
- In experiments, anidulafungin showed significant fungicidal activity against various Candida isolates, with impressive results at high concentrations (MICs) resulting in effective cell kill and prolonged inhibition of regrowth for over 12 hours.
- The drug also caused changes in cell wall structure and reduced the ability of Candida to adhere to human cells, suggesting that its effectiveness comes not only from killing the fungi directly but also by making it harder for the pathogens to infect and survive in hosts.
Article Abstract
Anidulafungin targets the cell walls of Candida species by inhibiting beta-1,3-glucan synthase, thereby killing isolates and exerting prolonged postantifungal effects (PAFEs). We performed time-kill and PAFE experiments on Candida albicans (n = 4), C. glabrata (n = 3), C. parapsilosis (n = 3), and C. krusei (n = 2) isolates and characterized the PAFEs in greater detail. MICs were 0.008 to 0.125 microg/ml against C. albicans, C. glabrata, and C. krusei and 1.0 to 2.0 microg/ml against C. parapsilosis. During time-kill experiments, anidulafungin caused significant kills at 16x MIC (range, log 2.68 to 3.89) and 4x MIC (log 1.87 to 3.19), achieving fungicidal levels (>or=log 3) against nine isolates. A 1-hour drug exposure during PAFE experiments resulted in kills ranging from log 1.55 to 3.47 and log 1.18 to 2.89 (16x and 4x MIC, respectively), achieving fungicidal levels against four isolates. Regrowth of all 12 isolates was inhibited for >or=12 h after drug washout. Isolates of each species collected 8 h after a 1-hour exposure to anidulafungin (16x and 4x MIC) were hypersusceptible to sodium dodecyl sulfate (0.01 to 0.04%) and calcofluor white (40 microg/ml). Moreover, PAFEs were associated with major cell wall disturbances, as evident in electron micrographs of viable cells, and significant reductions in adherence to buccal epithelial cells (P
Download full-text PDF
Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715634 PMC http://dx.doi.org/10.1128/AAC.01480-08 DOI Listing Publication Analysis
Top Keywords
Similar Publications
Dereplication of Natural Product Antifungals via Liquid Chromatography-Tandem Mass Spectrometry and Chemical Genomics.
Molecules
December 2024
Pharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, WI 53705, USA.
Recently expanded reports of multidrug-resistant fungal infections underscore the need to develop new and more efficient methods for antifungal drug discovery. A ubiquitous problem in natural product drug discovery campaigns is the rediscovery of known compounds or their relatives; accordingly, we have integrated Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for structural dereplication and Yeast Chemical Genomics for bioprocess evaluation into a screening platform to identify such compounds early in the screening process. We identified 450 fractions inhibiting and the resistant strains of and among more than 40,000 natural product fractions.
View Article and Find Full Text PDFDistribution and antifungal susceptibility of species causing vulvovaginal candidiasis and urinary tract infection in Medlatec healthcare system, Ha Noi city, Vietnam in 2023.
Ther Adv Infect Dis
January 2025
Department of Medical Parasitology, Military Medical University, No. 160 Phunghung Road, Hadong District, Hanoi 100000, Vietnam.
Background: Vulvovaginal candidiasis and urinary tract infections caused by are common diseases. While the most common causative agent is , other species, such as non-, can also be responsible. Susceptibility to antifungal drugs varies among species, but there is very limited information available from Vietnam.
View Article and Find Full Text PDFIdentification of spp. From the Gastrointestinal Tract of Pet Parrots and Subsequent Antifungal Susceptibility Profiles.
J Avian Med Surg
January 2025
Pathology and Wildlife Laboratory, Federal University of Acre, Rio Branco, Acre, 69920-900, Brazil.
Psittaciformes kept as pets can serve as reservoirs of various microorganisms, many of which have zoonotic potential, including spp. In this study, the antifungal susceptibility profiles of 16 spp. isolated from the oral and cloacal cavities of 20 pet parrots were evaluated.
View Article and Find Full Text PDFFluconazole-Resistant Vulvovaginal Candidosis: An Update on Current Management.
Pharmaceutics
December 2024
Femicare, Clinical Research for Women, 3300 Tienen, Belgium.
Currently, the rising prevalence of resistant species, particularly , as well as non-albicans isolates such as and , represent challenges in their management. In this review, we aimed to explore the current management of fluconazole-resistant vulvovaginal candidiasis (FRVVC). Identified studies focused on alternative antifungal therapies, including boric acid, nystatin, and newer agents like oteseconazole and ibrexafungerp.
View Article and Find Full Text PDFmeningitis in a patient with newly diagnosed acquired immunodeficiency syndrome from Sikkim, India.
Curr Med Mycol
May 2024
Department of Microbiology, Sikkim Manipal Institute of Medical Science, Gangtok, India.
Background And Purpose: infections in India have shifted, with an increase in the incidence rate of invasive candidiasis, particularly due to non- species. The central nervous system infections by are sparsely reported and more understanding and research is needed regarding these infections.
Case Report: This study reported an unusual case of meningitis in a middle-aged female with pulmonary tuberculosis and newly diagnosed acquired immunodeficiency syndrome with a low cluster of differentiation 4 count (12 cells/mm).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!